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Related Experiment Video

Updated: May 5, 2026

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget

Published on: November 20, 2018

An alternative inhibitory avoidance task for studying hippocampus-dependent spatial aversive memory in mice.

Haiyan Wang^1,2,3, Masanori Nomoto^4,5,6,7, Emi Murayama^1,2,3

¹Research Center for Idling Brain Science, University of Toyama, Toyama, 930-0194, Japan.

Molecular Brain

|May 3, 2026

Summary

Mice learned to avoid a preferred path after experiencing aversive air puffs. Stronger avoidance was linked to repeated air puff exposure, and hippocampal activity was crucial for recalling this spatial aversion memory.

Keywords:

Alternative inhibitory avoidance Hippocampus Route choice Spatial aversive memory Spatial navigation

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Last Updated: May 5, 2026

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Published on: July 29, 2025

Area of Science:

Neuroscience
Behavioral Science

Background:

Adaptive route choice is vital for survival, requiring navigation around dangers.
Existing mouse models for studying avoidance behavior during navigation are limited.
Neural mechanisms of experience-dependent route choice modification are not fully understood.

Purpose of the Study:

To establish a novel behavioral task for assessing avoidance-based route choice modification in mice.
To investigate how aversive experiences alter previously learned path preferences.
To determine the role of hippocampal activity in retrieving spatial aversive memories.

Main Methods:

Developed an air puff-based alternative inhibitory avoidance (AIA) task.
Trained mice to prefer a short path for water reward, then introduced air puffs to deter path use.
Assessed avoidance behavior at 6-h and 24-h intervals.
Used chemogenetics to suppress hippocampal activity before memory retrieval tests.

Main Results:

Mice receiving only 3 air puffs showed weak avoidance.
Consistent air puff exposure led to strong avoidance behavior at 6-h and 24-h tests.
Suppression of hippocampal activity before testing impaired aversive memory retrieval.

Conclusions:

The AIA task is a valuable paradigm for studying experience-dependent route choice modification due to aversive events.
Retrieval of spatial aversive memories in this task relies on hippocampal function.
This research sheds light on the neural basis of adaptive navigation strategies informed by negative experiences.