Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment.

Journal of visualized experiments : JoVE·2026
Same author

Retraction notice to "Decoding tumor microenvironment: EMT modulation in breast cancer metastasis and therapeutic resistance, and implications of novel immune checkpoint blockers" [Biomedicine & Pharmacotherapy 181 (2024) 117714].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Oxygen Vacancy-Engineered CaCu<sub>3</sub>Ti<sub>4</sub>O<sub>12</sub> Nanocatalyst for Piezoelectrically Driven Cascade Apoptosis/Cuproptosis/Ferroptosis Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

From online to offline: the impact of digital communication on activity participation among older adults.

Frontiers in public health·2026
Same author

Case report: A case of malignant phyllodes tumor of the breast with concurrent epithelial malignancy and heterologous chondrosarcomatous differentiation.

Frontiers in oncology·2026
Same author

Causal effects of maternal BMI on pregnancy outcomes: a Mendelian randomisation study investigating the mediating role of blood counts.

Frontiers in genetics·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

875

High-resolution confocal Raman microscopy spectrometer design based on echelle gratings.

Jian Zhang, Xiaotian Li, Jiri Jirigalantu

    Optics Express
    |May 4, 2026
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel confocal Raman spectrometer using an echelle grating, achieving high resolution and broad spectral coverage. The portable, modular system enhances signal collection for advanced material analysis and research.

    More Related Videos

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
    09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    3.6K
    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    12.6K

    Related Experiment Videos

    Last Updated: May 5, 2026

    A Multimodal Wide-Field Fourier-Transform Raman Microscope
    06:48

    A Multimodal Wide-Field Fourier-Transform Raman Microscope

    Published on: December 30, 2025

    875
    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
    09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    3.6K
    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    12.6K

    Area of Science:

    • Spectroscopy
    • Optical Engineering

    Background:

    • Conventional Raman spectrometers face limitations in balancing spectral coverage and resolution.
    • Existing designs often sacrifice throughput when aiming for higher resolution.

    Purpose of the Study:

    • To develop a cross-dispersed confocal Raman spectrometer with enhanced resolution and throughput.
    • To improve signal collection efficiency and signal-to-noise ratio for microscale analysis.

    Main Methods:

    • Incorporated a compound aberration correction module with cylindrical and spherical lenses.
    • Utilized a custom confocal Raman collection module for microscale focusing.
    • Employed an echelle grating (52.67 grooves/mm) as the primary dispersive element.
    • Connected components via optical fibers for portability and modularity.

    Main Results:

    • Achieved approximately double the resolution of traditional echelle grating spectrometers without compromising light throughput.
    • Reduced RMS spot radius to below 25 μm via aberration correction.
    • Demonstrated a high resolution of 1 cm⁻¹ (FWHM of 1.02 cm⁻¹ with Hg lamp) across a broad spectral range (70-4130 cm⁻¹).
    • Verified enhanced signal-to-noise ratio and characteristic peak accuracy through experimental validation.

    Conclusions:

    • The developed spectrometer offers a high-resolution solution for Raman spectroscopy.
    • The system is suitable for diverse applications including material analysis, biomedical research, and environmental monitoring.
    • Portability and modularity make it adaptable for both laboratory and field settings.