Identification of CYP450 Family Members and Their Gonadal Expression Profiles in Exopalaemon carinicauda

The cytochrome P450 (CYP450) superfamily plays important roles in a wide range of biological processes. The classification of CYP450 family members has been studied in some plants and animals; however, there are no reports on CYP450 family members in Exopalaemon carinicauda. Based on publicly available whole-genome data for E. carinicauda, we identified 58 CYP450 family members based on the genome-wide alignment and analyzed their domains, gene structures and chromosomal locations, as well as physicochemical properties of the encoded proteins. The results revealed that CYP450 family members, widely distributed across multiple chromosomes, exhibit diverse protein properties, gene structures, and conserved motifs. Phylogenetic analysis indicated that these members are primarily clustered into subfamilies 2, 3, and 4, and the mitochondrial clan, showing close genetic relationships with homologous genes from other crustaceans. In this study, we revealed the genetic structural characteristics of the CYP450 family in E. carinicauda. We identified candidate genes for future research on the molecular mechanisms of CYP450 in development and reproduction. These findings are expected to serve as a foundation for further studies in this field.