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Circular Approach to Biomanufacturing: Enhancing Therapeutic Protein Production Using Chum Salmon Head Peptone.

Svini Dileepa Marasinghe1,2, Minthari Sakethanika Bandara1,2, Somyong Lee3

  • 1Jeju Bio Research Center, Korea Institute of Ocean Science and Technology, 2670, Iljudong-ro, Gujwa-eup, Jeju 63349, Republic of Korea.

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Summary

Chum salmon head waste can be converted into a valuable peptone for microbiology. This sustainable chum salmon head peptone (CSHP) outperforms commercial options and reduces environmental impact.

Keywords:
Escherichia coli BL21(DE3)Oncorhynchus ketachum salmon head peptone (CSHP)expressionlife cycle assessment (LCA)recombinant protein

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Area of Science:

  • Marine biotechnology
  • Bioprocess engineering
  • Environmental science

Background:

  • Fish waste presents environmental and economic challenges for the marine food industry.
  • Sustainable valorization of fish waste is crucial for resource recovery and waste minimization.
  • Peptones are essential nitrogen sources in microbiological media, but traditional sources have limitations.

Purpose of the Study:

  • To evaluate the conversion of chum salmon head (CSH) waste into a high-value peptone for microbiological applications.
  • To characterize the chum salmon head peptone (CSHP) and compare its performance against commercial peptones.
  • To assess the environmental impact and biotechnological potential of CSHP.

Main Methods:

  • Hydrolysis of chum salmon head waste using various proteolytic enzymes, with Protamex yielding optimal results.
  • Characterization of the resulting chum salmon head peptone (CSHP), including molecular mass and amino nitrogen content.
  • Evaluation of CSHP as a nitrogen source for recombinant protein production in Escherichia coli and life cycle assessment.

Main Results:

  • Protamex enzyme treatment yielded the highest hydrolysis and recovery rates for CSH.
  • CSHP demonstrated superior characteristics (557 Da, 4.9% amino nitrogen) compared to commercial animal peptone (AP) and vegetable peptone (VP).
  • CSHP supported higher expression of human superoxide dismutase (hSOD) and human growth hormone (hGH) in E. coli and had a lower carbon footprint.

Conclusions:

  • Chum salmon head peptone (CSHP) is a sustainable and high-value alternative to conventional peptones.
  • CSHP offers significant potential for microbiological applications and recombinant protein production.
  • Utilizing CSHP contributes to marine waste reduction and circular bioeconomy strategies.