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Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
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Inflammatory bowel disease is a group of chronic disorders marked by recurrent inflammation of the gastrointestinal tract due to an abnormal immune response against gut microflora. This leads to tissue damage. The two main forms are Crohn’s disease and ulcerative colitis.Crohn’s DiseaseCrohn’s disease is a relapsing inflammatory disorder that can affect any part of the GI tract, from the mouth to the anus. It involves all layers of the bowel wall (transmural) and shows...
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Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
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Related Experiment Video

Updated: May 5, 2026

Murine Colitis Modeling using Dextran Sulfate Sodium DSS
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DSS Colitis Model: Traps, Tricks, and Reporting Recommendations.

Martina Perše1

  • 1Medical Experimental Centre, Institute of Pathology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.

Biomedicines
|May 4, 2026
PubMed
Summary

The dextran sodium sulfate (DSS) colitis model is widely used for inflammatory bowel disease (IBD) research. This review synthesizes factors influencing DSS colitis outcomes to improve reproducibility and translational relevance.

Keywords:
DSSbehaviourcolitiscytokinesgeneticgut microbiotagut–brain axisgut–liver axisimmune cellsinflammationreportingvisceral pain

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Area of Science:

  • * Gastroenterology and Immunology
  • * Experimental models of disease

Background:

  • * The dextran sodium sulfate (DSS) colitis model is a prevalent experimental tool for studying inflammatory bowel disease (IBD).
  • * Its widespread use, evidenced by thousands of publications, highlights its significance in understanding IBD pathogenesis.
  • * However, the model's inherent complexity and inconsistent reporting present challenges in study design, interpretation, and translational application.

Purpose of the Study:

  • * To synthesize the intrinsic and extrinsic factors that influence the dextran sodium sulfate (DSS) colitis model.
  • * To highlight the complexities and interdependencies within the DSS model for improved understanding and application.
  • * To provide practical guidance for enhancing reproducibility, interpretation, and translational value in DSS colitis research.

Main Methods:

  • * Comprehensive review of existing literature on the DSS colitis model.
  • * Synthesis of intrinsic factors including gut-brain and gut-liver axes.
  • * Analysis of extrinsic factors such as environmental, microbiological, and genetic influences.

Main Results:

  • * DSS colitis involves local inflammation that can impact systemic organs like the liver and brain, affecting metabolism and molecular responses.
  • * Extrinsic factors (environmental, microbiological, genetic) significantly influence experimental outcomes and data interpretation.
  • * Inconsistent reporting and methodological nuances complicate reproducibility and comparability across studies.

Conclusions:

  • * A thorough understanding of the interplay between intrinsic, extrinsic, and technical variables is crucial for accurate DSS model interpretation.
  • * Meticulous reporting and consideration of methodological details are essential for enhancing reproducibility and translational relevance.
  • * The review offers practical recommendations and a checklist to aid researchers in applying and interpreting DSS model results effectively.