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Related Experiment Video

Updated: May 5, 2026

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
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Histone Demethylase JMJD2D Suppresses Influenza A Virus Infection by Promoting RIG-I Expression.

Xiaochun Xia1, Jiadi Liang2, Hanshi Guo2

  • 1Department of Public Health and Medical Technology, Xiamen Medical College, Xiamen 361023, China.

Biomolecules
|May 4, 2026
PubMed
Summary

Histone demethylase JMJD2D is crucial for controlling Influenza A virus (IAV) infection. Upregulated JMJD2D enhances the antiviral response by boosting RIG-I expression, offering a potential therapeutic target for IAV.

Keywords:
JMJD2DRIG-Ihistone demethylaseinfluenza A virus

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Area of Science:

  • Immunology
  • Epigenetics
  • Virology

Background:

  • Influenza A virus (IAV) poses a significant global health threat.
  • The epigenetic regulation of host antiviral responses is not fully understood.
  • Rapid pathogen recognition and immune activation are key to controlling IAV.

Purpose of the Study:

  • To identify epigenetic regulators of the host antiviral response to IAV.
  • To investigate the role of histone demethylase JMJD2D in IAV infection.
  • To elucidate the mechanism by which JMJD2D influences antiviral signaling.

Main Methods:

  • Analysis of JMJD2D expression in clinical IAV infection samples.
  • Assessment of IAV susceptibility in JMJD2D-deficient mice.
  • In vitro studies involving JMJD2D knockdown and pharmacological inhibition in lung cells.
  • Investigation of epigenetic modifications at the RIG-I promoter region.

Main Results:

  • JMJD2D expression is upregulated during IAV infection.
  • JMJD2D-deficient mice show increased susceptibility to IAV, with higher viral loads and lung damage.
  • Inhibition of JMJD2D reduces IAV replication and innate immune response.
  • JMJD2D removes H3K9me3 at the RIG-I promoter, enhancing RIG-I expression via cooperation with NF-κB.

Conclusions:

  • JMJD2D is a critical epigenetic regulator of host defense against IAV.
  • JMJD2D enhances antiviral immunity by promoting RIG-I expression.
  • JMJD2D represents a potential therapeutic target for managing severe IAV infections.