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Synthesis, Structural Characterization and In Vitro Immunosuppressive Activity of Quinoa Bran Soluble Dietary
Hongyang Shu1,2, Yichen Ai1, Huajie Yin1
1College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Foods (Basel, Switzerland)
|May 4, 2026
Summary
Gallium chelation of soluble dietary fiber (SDF) from quinoa bran created a novel SDF-Ga complex. This complex demonstrated significant anticancer effects against colorectal cancer cells and modulated immune responses, suggesting potential for new nutraceuticals.
Area of Science:
- Biochemistry
- Immunology
- Nutraceutical Science
Background:
- Dietary fiber's biological effects are linked to its structure and immune interactions.
- Soluble dietary fiber (SDF) is a key component in functional foods.
- Quinoa bran is a potential source of valuable dietary fibers.
Purpose of the Study:
- To investigate the effects of gallium ion (Ga3+) chelation on soluble dietary fiber (SDF) from quinoa bran.
- To evaluate the immunomodulatory and anticancer properties of the resulting SDF-Ga complex.
- To explore the potential of SDF-Ga as a novel nutraceutical ingredient.
Main Methods:
- Soluble dietary fiber (SDF) from quinoa bran was chelated with gallium ions (Ga3+) to form SDF-Ga.
- Physicochemical properties of SDF-Ga, including molecular weight and polymer structure, were analyzed.
- In vitro assays were conducted using HCT-116 colorectal cancer cells and NK cells to assess cytotoxicity, proliferation, migration, and immune factor expression.
- Co-culture assays and cell viability assays were performed to evaluate anticancer efficacy.
Main Results:
- Gallium chelation altered SDF structure, reducing molecular weight and increasing chain branching, forming a compact 3D network.
- SDF-Ga significantly enhanced the cytotoxicity of NK cells against HCT-116 colorectal cancer cells (45.32% at 100 μg/mL).
- SDF-Ga demonstrated notable upregulation of key immune factors.
- Co-culture assays showed SDF-Ga inhibited HCT-116 cell proliferation and migration (p < 0.01).
- In vitro assays confirmed concentration-dependent inhibition of HCT-116 cell viability by SDF-Ga, showing enhanced anticancer potential over unmodified SDF.
Conclusions:
- Gallium chelation is an effective strategy for enhancing the functional properties of dietary fibers.
- The SDF-Ga complex exhibits promising dual immunomodulatory and anticancer activities.
- SDF-Ga holds potential for the development of novel nutraceuticals and health-promoting food products.
