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Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

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Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
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Microbes in Beverage Production01:25

Microbes in Beverage Production

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Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
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Microbes in Food Production01:29

Microbes in Food Production

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Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
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Microbial Spoilage of Food01:23

Microbial Spoilage of Food

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Microbial food spoilage refers to the degradation of food quality resulting from the metabolic activity of microorganisms such as bacteria, yeasts, and molds. These microbes proliferate on various food substrates depending on factors such as moisture content, nutrient availability, and storage conditions, leading to undesirable sensory and structural changes.Bacteria are primary agents of spoilage in high-moisture, nutrient-dense foods like meat, milk, and vegetables. Microbial spoilage occurs...
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Fermentation01:29

Fermentation

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Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
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Microorganisms in Agriculture and Food industry01:27

Microorganisms in Agriculture and Food industry

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Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
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Related Experiment Video

Updated: May 5, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
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Assessing Food Safety Risks in Homemade Fermented Beverages: A Case Study with Quinoa Rejuvelac.

Cristiana Guimarães Brasileiro1, Marcos Thalyson da Conceicao Moreno1, Eidy de Oliveira Santos1

  • 1Department of Biology, Rio de Janeiro State University (UERJ), Rio de Janeiro 20550-013, RJ, Brazil.

Life (Basel, Switzerland)
|May 4, 2026
PubMed
Summary
This summary is machine-generated.

Spontaneous fermentation of rejuvelac, a quinoa drink, risks contamination. The study found environmental bacteria, not beneficial ones, indicating a need for better hygiene and controlled fermentation for consumer safety.

Keywords:
artisanal fermented beveragesfood safetyseed-borne endophytesspontaneous fermentation

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Area of Science:

  • Food microbiology
  • Fermentation science
  • Consumer safety

Background:

  • Spontaneous fermentation in artisanal beverages can lead to uncontrolled microbial growth and foodborne contamination risks.
  • Characterizing microbial communities is crucial for ensuring the safety of homemade fermented products like rejuvelac.

Purpose of the Study:

  • To investigate the microbial composition of quinoa-based rejuvelac.
  • To identify potential microbiological hazards linked to its spontaneous preparation.
  • To assess the safety of rejuvelac as a functional food.

Main Methods:

  • Utilized high-throughput sequencing of the 16S rRNA V3-V4 region for bacterial community profiling.
  • Employed shotgun metagenomics to recover metagenome-assembled genomes.
  • Analyzed microbial composition to identify dominant taxa and potential pathogens.

Main Results:

  • The study revealed a dominance of Pseudomonadales (e.g., Pseudomonas, Acinetobacter) and Burkholderiales.
  • Lactic acid bacteria, essential for safe fermentation, were notably absent.
  • Metagenomics identified non-beneficial taxa, indicating deviations from safe fermentation pathways.

Conclusions:

  • Spontaneous rejuvelac preparation favors environmental contaminants over beneficial fermentative microbes.
  • Highlights the critical need for enhanced hygiene, controlled starter cultures, and monitoring in artisanal fermented food production.
  • Emphasizes the importance of improved safety standards to mitigate risks and protect consumers.