Modulation of S100β and Inflammatory Signalling by Isorhamnetin Enhances Peripheral Nerve Regeneration.

Area of Science:

• Neuroscience
• Pharmacology
• Regenerative Medicine

Background:

• Peripheral nerve injury is a major cause of disability with limited effective treatments.
• Developing novel therapeutic compounds for rapid and complete functional recovery is crucial.

Purpose of the Study:

• To evaluate the efficacy of isorhamnetin (ISO) in promoting functional rehabilitation following sciatic nerve injury in a mouse model.
• To investigate ISO's impact on sensorimotor function, nerve regeneration, oxidative stress, and inflammation.

Main Methods:

• Sciatic nerve injury was induced in BALB/c mice, followed by intraperitoneal administration of ISO (10 mg/kg) or vehicle.
• Functional recovery was assessed using behavioral analyses.
• Biochemical assays measured oxidative stress markers and blood glucose.
• Muscle fiber structure and nerve regeneration markers (S100β, IL-6, TNF-α) were analyzed.

Main Results:

• ISO treatment led to significant sensorimotor function recovery (p < 0.001).
• A notable reduction in oxidative stress markers and blood glucose levels was observed.
• ISO administration improved muscle fiber cross-sectional area and downregulated S100β, TNF-α, and IL-6 expression.

Conclusions:

• Isorhamnetin (ISO) demonstrates significant potential in accelerating functional recovery after peripheral nerve injury.
• ISO exerts its therapeutic effects by modulating inflammatory and oxidative stress pathways, thereby promoting nerve regeneration.