Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

54
An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...
54
Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

44
Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.
44

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatiotemporal White Matter Pathology in Chronic Cerebral Hypoperfusion: Insights from the BCAS Mouse Model of Vascular Cognitive Impairment and Dementia.

Aging and disease·2026
Same author

Protective role of complement signaling in Kawasaki disease vasculitis.

JCI insight·2026
Same author

Tomatidine is a senotherapeutic compound that improves cognitive function and reduces cellular senescence in aged mice.

EMBO molecular medicine·2026
Same author

The Aging Microenvironment Is a Determinant of Immune Exclusion and Metastatic Fate in Pancreatic Cancer.

Cancer research·2025
Same author

SORLA upregulation suppresses global pathological effects in aged tauopathy mouse brain.

bioRxiv : the preprint server for biology·2025
Same author

Unveiling Genomic Rearrangements in Engineered iPSC Lines by Optical Genome Mapping.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: May 5, 2026

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
12:14

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

35.7K

Multimodal Single-Cell Transcriptomic and Chromatin Accessibility Profiling Reveals Monocyte-Derived Macrophage

Milton H Hamblin1, Rabi Murad2, Austin C Boese3

  • 1Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA 92521, USA.

International Journal of Molecular Sciences
|May 4, 2026
PubMed
Summary

Stroke triggers brain monocyte recruitment and differentiation into monocyte-derived macrophages (MDMs). A specific MDM subset emerges, enhancing debris clearance and supporting early stroke recovery by utilizing lysosomal and lipid-processing functions.

Keywords:
ischemic strokemonocyte-derived macrophagessingle-cell chromatin profilingsingle-cell transcriptomics

More Related Videos

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

575
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

11.1K

Related Experiment Videos

Last Updated: May 5, 2026

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
12:14

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

35.7K
Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

575
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

11.1K

Area of Science:

  • Neuroscience
  • Immunology
  • Molecular Biology

Background:

  • Ischemic stroke causes monocyte infiltration and differentiation into monocyte-derived macrophages (MDMs) in the brain.
  • MDMs play a dual role in stroke, aiding debris clearance but potentially worsening neuroinflammation.
  • The heterogeneity and functional states of MDMs during the subacute stroke phase are not well understood.

Purpose of the Study:

  • To characterize the transcriptional and epigenetic landscape of MDM subsets in the subacute phase of ischemic stroke.
  • To identify specific MDM trajectories and regulatory mechanisms involved in post-stroke brain recovery.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) to analyze gene expression in mouse brain cells 48 hours after stroke.
  • Single-cell ATAC sequencing (scATAC-seq) to assess chromatin accessibility in stroke-affected brain regions.
  • Bioinformatic analysis including cell clustering and trajectory inference.

Main Results:

  • Identification of distinct monocyte-derived macrophage (MDM) subsets, including a Cd68hi/Ctsdhi subset with high lysosomal and lipid-processing gene expression.
  • Cell trajectory analysis revealed a transition from early recruited MDMs to the Cd68hi/Ctsdhi state, promoting a clearance-competent phenotype.
  • scATAC-seq data showed cell type-specific chromatin remodeling and regulatory element accessibility in key lipid metabolism and lysosomal function genes within MDMs.

Conclusions:

  • A detailed cellular and regulatory framework of the subacute post-stroke brain environment was established.
  • A specific trajectory towards a lysosome-enriched Cd68hi/Ctsdhi MDM phenotype was identified, crucial for early stroke recovery.
  • Endolysosomal and lipid-processing pathways in MDMs are highlighted as critical targets for promoting stroke recovery.