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Published on: October 5, 2016
Combined Transcriptome and Metabolome Analysis of the Quality Change Mechanism of the 'Pingguoli' Pear with a
Yuying Li1,2, Xiangyi Wang1,2, Yimo Yang1,2
1Department of Horticulture and Landscape Architecture, College of Agriculture, Yanbian University, Yanji 133002, China.
Abstract:
The 'Pingguoli' pear (Pyrus pyrifolia cv. Pingguoli) has a cultivation history spanning nearly one hundred years. Bud mutation selection is an important breeding method for the 'Pingguoli' pear. In this study, high-throughput sequencing technology (RNA-Seq) and non-targeted metabolomics (LC-MS/MS) were used to analyze the large-fruited bud mutation line (LFS) and normal type (NTF) of the 'Pingguoli' pear during the cell division (G1), rapid growth (G2), and mature stages (G3) of the fruit. The results showed that LFS exhibited a 46.32% increase in average single fruit weight (383.01 ± 54.72 g vs. 261.76 ± 10.79 g, p < 0.01) and a 19.10% decrease in soluble solids content (12.70 ± 0.94% vs. 15.40 ± 2.06%, p < 0.05) compared to NTF. Compared with the NTF, the content of total phenols and total flavonoids and the activity of antioxidant enzymes in the LFS fruits were significantly higher, while the contents of soluble sugar, reducing sugar, and soluble protein were significantly lower. Transcriptome analysis revealed that key metabolic pathways-including pentose and glucuronate interconversions, starch and sucrose metabolism, and cutin, suberine, and wax biosynthesis-were significantly enriched between NTF and LFS. These pathways may contain the specific differentially expressed genes (e.g., those involved in sugar metabolism and wax biosynthesis) identified as potential regulators of fruit size, appearance, and nutritional quality in the LFS. LC-MS/MS analysis identified key differentially accumulated metabolites, including L-arginine, caffeic acid, L-cysteine, pyridoxamine 5'-phosphate, adenosine-5'-phosphosulfate, neopentolactone D, chlorogenic acid, and gluconic acid, which are directly associated with the nutritional and antioxidant differences between LFS and NTF. The genes most related to metabolites in the three different developmental periods of the LFS and NTF were identified through combined analysis. These results provide insights for further research on bud mutation breeding and the quality formation mechanism of 'Pingguoli' pears.
