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Updated: May 5, 2026
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Detection of Dengue Virus Serotype 3 Using a Colorimetric Reverse Transcription Loop-Mediated Isothermal
Perla Pérez-Tepos1,2, Gilma Guadalupe Sánchez-Burgos3, Beatriz Xoconostle-Cázares2
1Unidad de Investigación Biomédica Oncológica Genómica, Hospital Gineco Pediatría 3A, OOAD Cd Mx Norte, Instituto Mexicano del Seguro Social, Ciudad de Mexico 07760, Mexico.
Researchers developed a new colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Dengue virus type 3 (DENV-3). This affordable diagnostic tool shows promise for resource-limited areas and point-of-care use.
Area of Science:
- Virology
- Molecular Biology
- Public Health
Background:
- Dengue virus (DENV) is a growing global health threat, necessitating accessible diagnostics, especially in resource-limited regions.
- Current diagnostic methods for DENV can be costly and require specialized equipment, hindering widespread use in endemic areas.
Purpose of the Study:
- To develop and validate a cost-effective, colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for Dengue virus type 3 (DENV-3) detection.
- To assess the assay's performance using clinical samples from Dengue-affected regions.
Main Methods:
- Design of primers targeting the 3' untranslated region (3' UTR) of DENV-3.
- Standardization of assay conditions for the colorimetric RT-LAMP.
- Validation using 95 previously diagnosed clinical samples from Southeastern Mexico.
Main Results:
- The developed colorimetric RT-LAMP assay demonstrated a preliminary limit of detection of 1 × 10^3 copies per reaction.
- The assay achieved 90.7% sensitivity and 100% specificity for DENV-3 detection.
- The colorimetric format allows for visual readout, eliminating the need for specialized equipment.
Conclusions:
- The colorimetric RT-LAMP assay is a viable tool for rapid DENV-3 screening and triage in resource-limited settings.
- This assay's ease of use and affordability support its potential application in point-of-care diagnostics.
- The developed method can aid in timely public health interventions and confirmatory testing.
