Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Inflammatory Bowel Disease III: Crohn's Disease

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Refractory Psoriatic Lesions in Japanese Patients With Biologics.

The Journal of dermatology·2026

Same author

Acid ceramidase overactivity drives ceramide loss, leading to atopic dry skin and Th2-skewed immune polarization.

The Journal of pathology·2026

Same author

Real-World Safety Profile of Spesolimab in Generalized Pustular Psoriasis: Insights From Japan as Part of a Multinational Expanded Access Program (EAP).

The Journal of dermatology·2025

Same author

A Novel Chimeric Molecule of Heparanase and Ig-Fc Enables Histochemical and Cytochemical Detection of <i>O</i>-sulfated Heparan Sulfate.

International journal of molecular sciences·2025

Same author

A Case of Palmoplantar Psoriasis Successfully Treated With Tapinarof Cream.

The Journal of dermatology·2025

Same author

Updated Analysis of Albinism in Japan: 290 Families With Novel Pathological Variants.

Pigment cell & melanoma research·2025

Search research articles

Related Experiment Video

Updated: May 5, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Immunohistochemistry Revealed Distinct IL-36γ Localization Among Different Skin Diseases.

Megumi Kishimoto¹, Miho Kimura-Sashikawa¹, Mayumi Komine¹

¹Department of Dermatology, Jichi Medical University, Tochigi, Japan.

The Journal of Dermatology

|May 4, 2026

Summary

Interleukin-36 gamma (IL-36γ) is elevated in psoriatic skin, but its expression and nuclear localization vary across inflammatory skin diseases. This suggests distinct pathogenic roles and potential disease-specific patterns for IL-36γ.

Keywords:

Interleukin‐36 generalized pustular psoriasis immunohistochemistry psoriasis pyoderma gangrenosum

More Related Videos

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

Skin Biopsy for Diagnosing Discoid Lupus Erythematosus

Skin Biopsy for Diagnosing Discoid Lupus Erythematosus

Published on: June 10, 2025

Related Experiment Videos

Last Updated: May 5, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

Skin Biopsy for Diagnosing Discoid Lupus Erythematosus

Skin Biopsy for Diagnosing Discoid Lupus Erythematosus

Published on: June 10, 2025

Area of Science:

Immunodermatology
Molecular Dermatology
Inflammatory Skin Diseases

Background:

Interleukin-36 gamma (IL-36γ) is implicated in psoriasis pathogenesis, driving neutrophil recruitment.
The precise localization of IL-36γ within psoriatic epidermis and its differential expression in other inflammatory skin conditions are not fully understood.

Purpose of the Study:

To investigate and compare the epidermal localization and nuclear positivity of IL-36γ in various inflammatory skin diseases, including psoriasis vulgaris and generalized pustular psoriasis.
To determine if IL-36γ expression patterns can differentiate between psoriasis and other skin conditions.

Main Methods:

Immunohistochemistry was employed to analyze IL-36γ expression in skin biopsies from patients with psoriasis vulgaris, generalized pustular psoriasis, pyoderma gangrenosum, atopic dermatitis, and other inflammatory skin diseases.
Staining intensity, distribution, and nuclear positivity of IL-36γ were quantitatively assessed and compared across disease groups.

Main Results:

IL-36γ was strongly expressed in the upper epidermis of psoriasis vulgaris and generalized pustular psoriasis, with significantly higher scores than in atopic dermatitis and normal skin.
Expression levels in pyoderma gangrenosum, palmoplantar pustulosis, and drug-induced eruptions were comparable to psoriasis.
Nuclear IL-36γ staining was prevalent in psoriasis (92-100%) but absent in pyoderma gangrenosum, a statistically significant difference.

Conclusions:

IL-36γ is upregulated in various inflammatory skin diseases, not exclusively psoriasis.
The distinct pattern of nuclear IL-36γ localization in psoriasis compared to pyoderma gangrenosum suggests potential disease-specific roles.
Further research is needed to elucidate the biological significance and mechanisms underlying IL-36γ's nuclear localization and its implications in skin inflammation.