Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybrid Zones02:29

Hybrid Zones

16.3K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
16.3K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

17.3K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
17.3K
In-situ Hybridization02:31

In-situ Hybridization

9.2K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.2K
Southern Blot02:57

Southern Blot

14.9K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variation in thermal plasticity of larval morphology among crested newt species and their reciprocal hybrids (Salamandridae: Triturus).

Journal of thermal biology·2026
Same author

Target Capture Sequencing Provides Insights Into Hybridogenetic Water Frogs.

Ecology and evolution·2026
Same author

Five Hidden Species in a Widespread European Vertebrate: Disentangling the Alpine Newt Cryptic Species Complex Through Genomic Phylogeography.

Molecular ecology·2026
Same author

Influence of Artificial Light at Night on Thyroid Gland Histology in <i>Triturus</i> Newts (Urodela, Salamandridae).

Animals : an open access journal from MDPI·2026
Same author

Cigarette smoking exposure and clinical outcomes in Graves' disease.

The Journal of clinical endocrinology and metabolism·2026
Same author

Triploidy in a pair of hybridizing salamanders at the far end of the speciation continuum.

The Journal of heredity·2026

Related Experiment Video

Updated: May 5, 2026

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

14.0K

Hybrid Horizons: Screening Hybridisation Through Nuclear Environmental DNA.

Daniel Zumel1, Emilie Didaskalou1, Tijana Vučić2,3,4

  • 1Institute of Environmental Sciences, Leiden University, Leiden, the Netherlands.

Molecular Ecology Resources
|May 4, 2026
PubMed
Summary

Environmental DNA (eDNA) can now screen for hybrid populations. This non-invasive method uses nuclear eDNA to track hybrid zones, offering a fast and cost-efficient conservation tool.

Keywords:
TriturusKASP genotypingcrested newtsnon‐invasive monitoringnuclear eDNAproof‐of‐concept

More Related Videos

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes
11:24

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Published on: December 5, 2025

388
An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model
06:05

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

Published on: March 9, 2022

3.8K

Related Experiment Videos

Last Updated: May 5, 2026

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

14.0K
Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes
11:24

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Published on: December 5, 2025

388
An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model
06:05

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

Published on: March 9, 2022

3.8K

Area of Science:

  • Evolutionary Biology
  • Conservation Genetics
  • Environmental Science

Background:

  • Monitoring hybrid zones is crucial for understanding evolution and preventing species loss.
  • Traditional methods for hybrid zone monitoring are often impractical due to cost, time, and difficulty sampling rare species.
  • Nuclear environmental DNA (eDNA) is scarce but essential for hybridisation studies, with its application previously untested.

Purpose of the Study:

  • To empirically validate the use of nuclear eDNA for screening hybrid populations.
  • To develop and test an eDNA-based toolkit for genotyping nuclear single nucleotide polymorphisms (SNPs) without sequencing.
  • To assess the concordance between eDNA-derived ancestry estimates and individual genotypes in a controlled setting.

Main Methods:

  • Developed an eDNA toolkit using Kompetitive Allele-Specific PCR (KASP) to genotype species-diagnostic nuclear SNPs.
  • Quantified ancestry levels by mapping fluorescence data to a hybrid index.
  • Compared eDNA-derived ancestry estimates with skin-swab genotypes from crested newts (Triturus ivanbureschi and T. macedonicus) and their F1 hybrids in mesocosms.

Main Results:

  • Demonstrated strong concordance between eDNA-based ancestry estimates and individual genotypes.
  • The eDNA approach proved effective across different eDNA sampling concentrations.
  • Successfully validated nuclear eDNA as a viable tool for hybrid population screening.

Conclusions:

  • Nuclear eDNA analysis, coupled with KASP genotyping, provides a powerful, non-invasive method for hybrid zone screening.
  • This toolkit offers a fast, cost-efficient, and scalable complement to conventional methods for biodiversity monitoring and conservation.
  • The approach is well-suited for locating and tracking hybrid zones, aiding in evolutionary and conservation efforts.