Related Experiment Video
Updated: May 5, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Influence of green tea-enriched fermentation and cold storage on yogurt's probiotic viability and metabolomic
Moumika Rahman Brishti1, Noor Liana Mat Yajit1,2, Ahmad Salihin Baba1
1Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Abstract:
This study investigated the synergistic effects of varying green tea powder concentrations and storage conditions on total phenolic content, antioxidant activity, microbial viabilityand the metabolite profile of yogurt formulations. The total phenolic content and antioxidant activity increased proportionally to the concentration of green tea powder. Enhanced acidity, faster fermentation, and higher Lactobacillus spp. viability was observed.Multivariate analysis demonstrated distinct group clustering, indicating clear metabolic divergence driven by the incorporation of green tea and fermentation in cold storage. A total of 86 metabolites were identified, which predominantly comprise polyphenols derived from green tea powder. Pathway enrichment analysis revealed that the synergistic interaction between LAB (lactic acid bacteria) and tea polyphenols during cow's milk fermentation significantly modulated multiple metabolic pathways, including central carbon metabolism, nucleotide metabolism, redox regulation, amino acid metabolism, and lipid metabolism, which are responsible for contributing enhanced nutritional and functional properties of yogurt.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-026-02138-4.
Related Concept Videos
Microbes in Food Production
Microbes in the Production of Fermented Foods
Probiotics
Microbes in Beverage Production
Microbial Spoilage of Food
Microbial Fermentation

