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Comprehensive safety assessment of Pediococcus acidilactici SY23 for potential probiotic use in inflammation

Soobin Park1, Huijin Jeong1, Na-Kyoung Lee2

  • 1Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.

Food Science and Biotechnology
|May 4, 2026
PubMed

Insights

Lactobacillus acidilactici SY23 was evaluated for probiotic safety. It demonstrated no harmful activities and possessed antibiotic susceptibility, suggesting its suitability as a safe probiotic candidate.

Area of Science:

  • Microbiology
  • Probiotics
  • Food Safety

Background:

  • Previous research indicated anti-inflammatory properties of Lactobacillus acidilactici SY23 in cell models.
  • A thorough safety assessment is crucial for probiotic strain validation.

Purpose of the Study:

  • To comprehensively evaluate the safety of Lactobacillus acidilactici SY23 for probiotic applications.
  • To confirm its suitability as a potential anti-inflammatory probiotic.

Main Methods:

  • In vitro assays for mucin degradation, gelatin liquefaction, urease, beta-glucuronidase, and bile salt deconjugation.
  • Analysis of biogenic amines, indoles, D-lactate production, beta-hemolytic activity, and cytotoxicity.
  • Antibiotic susceptibility testing and whole-genome sequencing.

Main Results:

  • Lactobacillus acidilactici SY23 showed no undesirable enzymatic activities or biogenic amine production.
  • No cytotoxicity or beta-hemolytic activity was observed. D-lactate was produced at ~47 mM.
  • All tested antibiotics showed minimum inhibitory concentrations below European Food Safety Authority (EFSA) cut-off values, with no acquired resistance genes detected.

Conclusions:

  • Lactobacillus acidilactici SY23 exhibited a favorable safety profile in vitro.
  • The absence of virulence factors and antibiotic resistance genes supports its probiotic potential.
  • Lactobacillus acidilactici SY23 is a promising candidate for probiotic development.
Keywords:
Anti-inflammatoryLactic acid bacteriaPediococcus acidilacticiProbioticsSafety assessment

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