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Updated: May 5, 2026
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
Plasmolyzed yeast cell system for the encapsulation of Pulicaria odora polyphenols: RSM-based process optimization
Djamel Eddine Laib1, Imen Laib1,2, Hamdi Bendif3
1Department of Agronomic Sciences, Faculty of Sciences, August 20, 1955 University, Skikda, Algeria.
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This study focused on optimizing the encapsulation of polyphenols extracted from Pulicaria odora into plasmolyzed Saccharomyces cerevisiae yeast cells using RSM. The polyphenols were obtained via ethanolic maceration, and encapsulation performance was evaluated by varying two key factors: the yeast-to-extract ratio and incubation time. A CCD was employed to model the process and determine optimal conditions. The model predicted maximum encapsulation efficiencies of 95.83% for total polyphenols and 96.83% for total flavonoids. The microcapsules obtained under these optimized conditions were further characterized to evaluate their structural, chemical, and functional properties. SEM confirmed the incorporation of bioactives into the yeast matrix, while FTIR indicated molecular interactions between polyphenols and yeast cell wall components. DSC revealed enhanced thermal stability of the encapsulated forms. Antioxidant activity was significantly improved after encapsulation. The findings highlight the potential of plasmolyzed yeast cells as natural, biocompatible carriers for stabilizing and delivering plant-derived bioactives for several applications.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-026-02128-6.
