Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
MicroRNAs01:22

MicroRNAs

9.8K
9.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Abnormal Proliferation02:23

Abnormal Proliferation

4.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.1K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Androgen Receptor Modulators: A Review of Their Therapeutic Promise and Concerns of Misuse.

Current sports medicine reports·2026
Same author

Perspective: use of beef in a dietary intervention as an effective strategy for improving cognition in young adult females.

Frontiers in nutrition·2026
Same author

Obesity and cancer: Methodological frontiers for mechanistic discoveries.

PLoS medicine·2026
Same author

Differential regulation of mitochondrial quality control in skeletal muscle by HZE radiation exposure and partial weightbearing in mice.

Life sciences in space research·2026
Same author

Daily mango (<i>Mangifera indica</i> L.) consumption supplemented with probiotics differentially modulates inflammation and cognitive function in individuals with overweight or obesity: a placebo-controlled, double-blind, and randomized trial.

Food & function·2025
Same author

ATG4B is required for mTORC1-mediated anabolic activity and is associated with clinical outcomes in non-small cell lung cancer.

FEBS open bio·2025

Related Experiment Video

Updated: May 5, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

6.8K

MicroRNA 15a and 16 Regulate Proteostasis in Non-Small Cell Lung Cancer.

Patrick J Ryan1, Bethany C Guerra1, Peter Nghiem2

  • 1Muscle Biology Laboratory, Department of Kinesiology and Sports Management Texas A&M University College Station Texas USA.

FASEB Bioadvances
|May 4, 2026
PubMed
Summary

MicroRNAs miR15a/16 regulate protein anabolism in non-small cell lung cancer (NSCLC). This discovery identifies novel metabolic regulators and potential therapeutic targets for lung cancer treatment.

Keywords:
anabolismcancermetabolismmicroRNAproteostasis

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.5K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.1K

Related Experiment Videos

Last Updated: May 5, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

6.8K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.5K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.1K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Aberrant anabolic activity is a hallmark of cancer, yet its regulators in cancer remain incompletely understood.
  • MicroRNAs (miRNAs) are small regulatory molecules with potential roles in maintaining protein homeostasis (proteostasis).

Purpose of the Study:

  • To investigate the role of co-transcribed miR15a and miR16 (miR15a/16) in regulating protein metabolism and pathophysiology in non-small cell lung cancer (NSCLC).

Main Methods:

  • Utilized cellular models of NSCLC.
  • Transfected cells with miR15a/16 mimetics.
  • Analyzed gene regulation in metabolic and pathological pathways.
  • Assessed cell growth and protein synthesis rates.

Main Results:

  • miR15a/16 were found to regulate genes involved in protein metabolism.
  • Transfection with miR15a/16 mimetics led to decreased cell growth in NSCLC models.
  • Protein synthesis rates were reduced following miR15a/16 mimic transfection.

Conclusions:

  • miR15a/16 function as regulators of protein anabolism in NSCLC.
  • These miRNAs represent novel metabolic regulators with potential as therapeutic targets for lung cancer.