Layered Enzymatic Biosensor Decorated with Prussian Blue Structures for Continuous Electrochemical Glucose Sensing

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Nanotechnology

Background:

• Diabetes mellitus management requires effective continuous glucose monitoring (CGM).
• Existing CGM technologies face challenges in accuracy, invasiveness, and real-time data acquisition.
• Electrochemical enzymatic biosensors offer a promising avenue for improved glucose detection.

Purpose of the Study:

• To develop an innovative electrochemical enzymatic biosensor for continuous glucose monitoring.
• To engineer a multilayered biosensor architecture for enhanced performance.
• To evaluate the biosensor's sensitivity, stability, and real-time monitoring capabilities.

Main Methods:

• Immobilization of glucose oxidase on a cystamine-modified ENIG electrode.
• Decoration of the electrode with spherical Prussian Blue nanostructures (30-90 nm).
• Chronoamperometric analysis to assess current responses to varying glucose concentrations.

Main Results:

• The multilayered architecture enhanced electron transfer efficiency and chronoamperometric stability.
• The biosensor exhibited immediate, reproducible current changes with superior sensitivity at 5 mM Prussian Blue.
• Stability tests over 8 days showed consistent performance, indicating potential for regeneration.
• Real-time monitoring in simulated interstitial fluid demonstrated sustained functionality and rapid signal response.

Conclusions:

• The developed electrochemical enzymatic biosensor shows significant potential for minimally invasive continuous glucose monitoring.
• The innovative multilayered design with Prussian Blue nanostructures improves sensor performance.
• Further development may lead to advanced diabetes management systems.