Study on plasma exosome miRNA sequencing and analysis in rats under hemorrhagic stress

This study explored the impact of hemorrhagic stress on the expression of plasma exosome miRNAs in rats using high-throughput miRNA sequencing technology. The results revealed that hemorrhagic stress significantly altered the expression profiles of multiple miRNAs, particularly the upregulation of miRNA-193b-3p, which is associated with vascular repair, and the downregulation of miRNA-485-5p, which may be related to impaired cell repair and antioxidant responses. Further target gene prediction and pathway analysis suggested that the differentially expressed miRNAs play potential roles in key physiological processes such as immune response, cell repair, and angiogenesis, especially in the enrichment of the TNF and MAPK signaling pathways. These findings suggest that these miRNAs may play important roles in the recovery process following hemorrhage by regulating these pathways. These results provide new evidence for the biological functions of miRNAs in hemorrhagic stress and offer potential biomarkers and therapeutic targets for early diagnosis and intervention. Although this study provides new insights into the role of exosome miRNAs in hemorrhagic stress, there are certain limitations, such as the use of only a rat model in the experiment. The broader applicability of the results requires further validation through other animal models or clinical studies.