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Related Experiment Video

Updated: May 5, 2026

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
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High-throughput screening to engineer optimal T cell therapies: current knowledge and future prospects.

Lei Liu1,2, Saisai Chen3, Meng Ding4

  • 1Department of General Surgery, Fuyang Hospital of Traditional Chinese Medicine, Fuyang, China.

Frontiers in Oncology
|May 4, 2026
PubMed
Summary

High-throughput screening advances cancer immunotherapy by identifying targets and substances to overcome resistance in adoptive T cell therapy (ACT). This accelerates the development of more effective ACT strategies for clinical application.

Keywords:
CRISPR screeningTCR-T cellsadoptive T cell therapiescellsgenetic screeninghigh-throughput screeningnon-genetic screening

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Area of Science:

  • Immunology
  • Biotechnology
  • Oncology

Background:

  • Adoptive T cell therapy (ACT) shows promise in cancer immunotherapy.
  • Clinical efficacy of ACT is frequently hindered by primary resistance.
  • Overcoming resistance is crucial for enhancing ACT effectiveness.

Purpose of the Study:

  • To review advancements in high-throughput screening (HTS) for optimizing ACT.
  • To highlight HTS applications in genetic and non-genetic screening relevant to ACT.
  • To provide guidance for future ACT research and clinical translation.

Main Methods:

  • Review of recent literature on high-throughput screening technologies.
  • Analysis of HTS applications in genetic screening of T cells and tumor cells.
  • Examination of non-genetic screening methods for small molecules and delivery systems.

Main Results:

  • HTS technologies are essential for identifying significant targets and substances.
  • HTS accelerates the development of advanced ACT strategies.
  • Applications span genetic screening and discovery of therapeutic agents.

Conclusions:

  • High-throughput screening is pivotal for overcoming resistance in ACT.
  • Continued advancements in HTS will drive innovation in cancer immunotherapy.
  • HTS offers valuable insights for future ACT research and clinical practice.