Related Experiment Video
Updated: May 5, 2026
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
Published on: March 18, 2020
High-throughput screening to engineer optimal T cell therapies: current knowledge and future prospects
Lei Liu1,2, Saisai Chen3, Meng Ding4
1Department of General Surgery, Fuyang Hospital of Traditional Chinese Medicine, Fuyang, China.
Abstract:
Adoptive T cell therapy (ACT) is a potent strategy in cancer immunotherapy, but its clinical efficacy is often limited by primary resistance. To overcome this challenge, high-throughput screening technologies have emerged as essential tools for optimizing ACT. By enabling the identification of biologically significant targets and substances from vast libraries, these technologies have accelerated the development of advanced ACT strategies. This review delves into the latest advancements in high-throughput screening, highlighting its applications in genetic screening of T cells and tumor cells, as well as non-genetic screening for small molecules and targeted delivery systems. These insights provide valuable guidance for future research and clinical applications of ACT.
Insights
High-throughput screening advances cancer immunotherapy by identifying targets and substances to overcome resistance in adoptive T cell therapy (ACT). This accelerates the development of more effective ACT strategies for clinical application.
Area of Science:
- Immunology
- Biotechnology
- Oncology
Background:
- Adoptive T cell therapy (ACT) shows promise in cancer immunotherapy.
- Clinical efficacy of ACT is frequently hindered by primary resistance.
- Overcoming resistance is crucial for enhancing ACT effectiveness.
Purpose of the Study:
- To review advancements in high-throughput screening (HTS) for optimizing ACT.
- To highlight HTS applications in genetic and non-genetic screening relevant to ACT.
- To provide guidance for future ACT research and clinical translation.
Main Methods:
- Review of recent literature on high-throughput screening technologies.
- Analysis of HTS applications in genetic screening of T cells and tumor cells.
- Examination of non-genetic screening methods for small molecules and delivery systems.
Main Results:
- HTS technologies are essential for identifying significant targets and substances.
- HTS accelerates the development of advanced ACT strategies.
- Applications span genetic screening and discovery of therapeutic agents.
Conclusions:
- High-throughput screening is pivotal for overcoming resistance in ACT.
- Continued advancements in HTS will drive innovation in cancer immunotherapy.
- HTS offers valuable insights for future ACT research and clinical practice.
