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Updated: May 5, 2026
11:19
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Published on: February 20, 2017
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Organelle partitioning in the multi-budding yeast Aureobasidium pullulans
Alison C E Wirshing1, Michelle Yan1, Daniel J Lew1
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
Biorxiv : the Preprint Server for Biology
|May 4, 2026
Summary
This study reveals how fungi distribute cellular components during multi-budding. While mitochondria and ER are evenly shared, vacuoles and peroxisomes show variable inheritance patterns in daughter cells.
Area of Science:
- Cell Biology
- Mycology
- Genetics
Background:
- Cellular organelle content is generally stable within a cell type, maintained by equitable partitioning during division.
- Most research on organelle inheritance focuses on binary fission, not complex division strategies.
Purpose of the Study:
- To characterize organelle inheritance in a fungus employing multi-budding, a division strategy producing multiple daughter cells per cycle.
- To investigate the distribution patterns of key organelles (mitochondria, ER, vacuole, peroxisomes) during fungal multi-budding.
Main Methods:
- Microscopy and live-cell imaging to track organelle distribution.
- Quantitative analysis of organelle inheritance in budding fungal cells.
Main Results:
- Mitochondria and endoplasmic reticulum (ER) were evenly distributed among all emerging daughter cells.
- Vacuoles and peroxisomes exhibited variable inheritance, with unequal distribution to daughter cells.
- The fungus produces 2–20 daughter cells in a single cell cycle via multi-budding.
Conclusions:
- Fungal multi-budding involves differential inheritance strategies for various organelles.
- Even inheritance of mitochondria and ER supports basic cellular function in all daughters.
- Variable inheritance of vacuoles and peroxisomes may confer adaptability in dynamic environments for this polyextremotolerant fungus.
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