Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

A Single-Component System01:24

A Single-Component System

69
In the field of chemistry, the terms "component" and "phase" hold significant importance. A component refers to a chemically distinct substance in a system that has specific properties. It is chemically homogeneous, meaning it has the same properties throughout. For example, in a mixture of salt and water, both salt and water are considered separate components because they have different chemical properties.On the other hand, a phase is a form of matter that has a consistent chemical...
69
State Space Representation01:27

State Space Representation

785
The frequency-domain technique, commonly used in analyzing and designing feedback control systems, is effective for linear, time-invariant systems. However, it falls short when dealing with nonlinear, time-varying, and multiple-input multiple-output systems. The time-domain or state-space approach addresses these limitations by utilizing state variables to construct simultaneous, first-order differential equations, known as state equations, for an nth-order system.
Consider an RLC circuit, a...
785
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
Control Systems: Applications01:25

Control Systems: Applications

1.2K
Electrical engineering plays a pivotal role in our daily lives, with control systems at the heart of many applications, from home appliances to sophisticated space shuttles. Control systems manage and regulate the behavior of devices and processes, ensuring they function safely, correctly, and efficiently.
In modern vehicles, control systems manage various functions to enhance performance and safety. The steering wheel and accelerator are primary inputs in a car's control system. The...
1.2K
Linear time-invariant Systems01:23

Linear time-invariant Systems

1.1K
A system is linear if it displays the characteristics of homogeneity and additivity, together termed the superposition property. This principle is fundamental in all linear systems. Linear time-invariant (LTI) systems include systems with linear elements and constant parameters.
The input-output behavior of an LTI system can be fully defined by its response to an impulsive excitation at its input. Once this impulse response is known, the system's reaction to any other input can be...
1.1K
Open and closed-loop control systems01:17

Open and closed-loop control systems

2.0K
Control systems are foundational elements in automation and engineering. They are broadly categorized into open-loop and closed-loop systems. These classifications hinge on the presence or absence of feedback mechanisms, significantly influencing the system's performance, complexity, and application.
An open-loop control system operates without feedback from the output. It consists of two primary elements: the controller and the controlled process. The controller receives an input signal...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: <i>Drosophila</i> FMRFa neuropeptide signaling modulates systemic glycogen metabolism and fitness in a sex-specific manner.

Frontiers in endocrinology·2026
Same author

<i>Drosophila</i> FMRFa neuropeptide signaling modulates systemic glycogen metabolism and fitness in a diet-dependent manner.

Frontiers in endocrinology·2026
Same author

Glucose-6-phosphatase is required for organelle reorganization, energy metabolism and motility of <i>Drosophila</i> sperm.

bioRxiv : the preprint server for biology·2025
Same author

<i>Drosophila</i> neuronal Glucose-6-Phosphatase is a modulator of neuropeptide release that regulates muscle glycogen stores via FMRFamide signaling.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

<i>Drosophila</i> Neuronal Glucose 6 Phosphatase is a Modulator of Neuropeptide Release that Regulates Muscle Glycogen Stores via FMRFamide Signaling.

bioRxiv : the preprint server for biology·2023
Same author

Opposing chemosensory functions of closely related gustatory receptors.

eLife·2023

Related Experiment Video

Updated: May 5, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

7.5K

Development of a novel simplified Q-system with integrated temporal expression control.

Ji-Eun Ahn1, Hubert Amrein1

  • 1Department of Cell Biology and Genetics, The Naresh K. Vashist College of Medicine, Texas A&M University, Bryan TX, 77845.

Biorxiv : the Preprint Server for Biology
|May 4, 2026
PubMed
Summary

Researchers developed a new temperature-controlled gene expression system for studying gene function. This intein-based Q-system allows precise temporal control of gene activity in various organisms, simplifying genetic research.

More Related Videos

Generation and Coherent Control of Pulsed Quantum Frequency Combs
06:42

Generation and Coherent Control of Pulsed Quantum Frequency Combs

Published on: June 8, 2018

9.0K
Interactive and Visualized Online Experimentation System for Engineering Education and Research
08:35

Interactive and Visualized Online Experimentation System for Engineering Education and Research

Published on: November 24, 2021

2.3K

Related Experiment Videos

Last Updated: May 5, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

7.5K
Generation and Coherent Control of Pulsed Quantum Frequency Combs
06:42

Generation and Coherent Control of Pulsed Quantum Frequency Combs

Published on: June 8, 2018

9.0K
Interactive and Visualized Online Experimentation System for Engineering Education and Research
08:35

Interactive and Visualized Online Experimentation System for Engineering Education and Research

Published on: November 24, 2021

2.3K

Area of Science:

  • Genetics and Molecular Biology
  • Developmental Biology
  • Gene Regulation

Background:

  • Bimodal gene expression systems are crucial for understanding gene, cell, and organ function.
  • Temporal control of gene expression has been a significant limitation in these systems.
  • The Q-system, utilizing the QF2 transcription factor, is widely used in model organisms like Drosophila.

Purpose of the Study:

  • To incorporate temperature-sensitive intein modules into the QF2 transcription factor for temporal control.
  • To develop a novel, temporally regulated gene expression system for broader applicability.
  • To overcome limitations of existing gene expression systems, particularly regarding temporal regulation.

Main Methods:

  • Integration of temperature-sensitive intein modules with distinct temperature profiles into QF2.
  • Testing the QF2_INTts activators in Drosophila larvae and adult flies under varying temperature conditions.
  • Utilizing QUAS reporters to monitor gene expression driven by the QF2_INTts system.

Main Results:

  • Temperature-sensitive QF2_INTts activators enable precise temporal control of QUAS reporter gene expression in Drosophila.
  • Permissive temperatures (18°C-25°C) activate QF2, driving reporter expression, while restrictive temperatures (23°C-30°C) inactivate it.
  • The system allows for both activation and deactivation of gene expression by switching between temperature conditions.
  • Animals expressing QUAS-Kir2.1 show viability under restrictive conditions, with temperature shifts leading to lethality or paralysis.

Conclusions:

  • The intein-based Q-system provides a robust and simplified method for temporal gene expression regulation, eliminating the need for drug-dependent suppressors.
  • QF2_INTts drivers offer significant advantages over the GAL4 system and can be implemented in diverse organisms, including insects and zebrafish.
  • This technology facilitates the study of gene and cell function across developmental stages and in various species, including disease vectors and poikilotherm vertebrates.