Related Experiment Video
Updated: May 5, 2026
10:26
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
Published on: January 20, 2019
12.3K
PABPC1 Modulates Immunoglobulin pre-mRNA Alternative Polyadenylation
Biorxiv : the Preprint Server for Biology
|May 4, 2026
Summary
Poly(A)-binding protein C1 (PABPC1) regulates gene expression by shortening 3' untranslated regions and downregulating target genes, particularly impacting B cell development and immunoglobulin secretion.
Area of Science:
- Molecular Biology
- Gene Regulation
- Immunology
Background:
- Alternative polyadenylation (APA) is crucial for tuning gene expression and its dysregulation is linked to disease.
- The precise role of Poly(A)-binding protein C1 (PABPC1) in gene regulation and APA is not well-defined.
- APA significantly influences immunoglobulin secretion during B cell development.
Purpose of the Study:
- To elucidate the function of PABPC1 in B cell development.
- To map PABPC1-RNA interactions and its impact on APA.
- To understand PABPC1's role in regulating gene expression.
Main Methods:
- Crosslinking, Ligation, and Sequencing (CLAP-seq) to map PABPC1-RNA interactions.
- Degron-based strategy to perturb PABPC1 expression.
- Integration with transcriptomic data and genomics modeling.
Main Results:
- PABPC1 binds to A-rich regions in the 3' UTR of 70% of its gene targets.
- PABPC1 downregulates approximately 60% of its target genes.
- PABPC1 binding is enriched in genes with shortened 3' UTRs and is associated with decreased gene expression, particularly near the polyadenylation site (PAS).
Conclusions:
- PABPC1 primarily functions to shorten 3' UTRs and downregulate gene expression in a context-dependent manner.
- PABPC1's role in immunoglobulin transcript APA and secretion is modest but specific.
- Findings clarify PABPC1's contribution to gene regulation via APA.
More Related Videos
11:02Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
7.6K
11:06Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
Published on: September 20, 2017
5.6K
Related Concept Videos
02:23Abnormal Proliferation
4.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
02:04Covalently Linked Protein Regulators
8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.2K
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
14.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
14.2K
02:23RNA Editing
8.3K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.3K
02:41Chromatin Structure Regulates pre-mRNA Processing
6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
6.6K
01:23Regulation of Expression at Multiple Steps
1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K