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Researchers developed novel organic afterglow imaging agents using coumarin derivatives. These activatable probes enable high-contrast bioimaging for diseases like ulcerative colitis and Parkinson's disease.

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Area of Science:

  • Organic chemistry
  • Biomedical imaging
  • Materials science

Background:

  • Afterglow luminescence imaging has shifted towards organic small molecules for bioimaging applications.
  • Developing flexible organic afterglow molecules with clear structure-luminescence relationships for high-contrast imaging is challenging.

Purpose of the Study:

  • To develop novel activatable organic afterglow probes for bioimaging.
  • To investigate the structure-luminescence relationship of coumarin derivatives for enhanced afterglow emission.
  • To demonstrate the application of these probes in assessing therapeutic efficacy.

Main Methods:

  • Synthesized coumarin derivatives and designed MP molecules with boronic ester and methylenecyclobutane moieties.
  • Constructed activatable afterglow probe C545-MP NPs by integrating coumarin and MP units.
  • Utilized oxidation by peroxynitrite (ONOO-) and subsequent irradiation to trigger afterglow emission.
  • Applied the probe for imaging in models of ulcerative colitis and Parkinson's disease.

Main Results:

  • Rigidified coumarin structures showed significantly stronger afterglow luminescence compared to flexible analogues.
  • The C545-MP NPs probe demonstrated bright afterglow emission upon activation.
  • The probe enabled high-contrast imaging by eliminating autofluorescence.
  • Successful assessment of therapeutic efficacy in ulcerative colitis and Parkinson's disease models was achieved.

Conclusions:

  • Coumarin derivatives can serve as effective organic afterglow luminescence materials.
  • The developed activatable probe C545-MP NPs offer a promising tool for high-contrast bioimaging.
  • This technology facilitates precise evaluation of therapeutic interventions for inflammatory and neurodegenerative diseases.