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Updated: May 5, 2026

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology
Published on: May 31, 2024
Sponge-assisted intracameral length titration: a new technical modification for Ab externo XEN implantation
Giovanni Rubegni1, Mario Fruschelli2, Karl Mercieca3
1Ophthalmology Unit, Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy. giovannirubegni@gmail.com.
Abstract:
Accurate positioning of the XEN gel stent is critical during ab externo implantation, and fine-tuning is commonly performed with forceps, which may concentrate stress on the gelatin implant. We describe a sponge-assisted swiping maneuver using sterile ocular sponge swabs as a soft interface to achieve gradual, controlled advancement or retraction of the stent. The technique enables stepwise titration of intracameral length while reducing focal compression and the risk of device deformation during repositioning. This low-cost modification can be readily incorporated into standard ab externo XEN workflows.

