Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipoteichoic acids from Streptococcus sanguis.

T H Chiu, L I Emdur, D Platt

    Journal of Bacteriology
    |May 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ancient mitogenomes of Phoenicians from Sardinia and Lebanon: A story of settlement, integration, and female mobility.

    PloS one·2018
    Same author

    Osteochondrosis lesions of the lateral trochlear ridge of the distal femur in four ponies.

    The Veterinary record·2011
    Same author

    The Effect of Long-Continued Suppressive Atabrine Medication on Relapses of Vivax Malaria.

    Transactions of the American Clinical and Climatological Association·2011
    Same author

    Vivax relapse rates following continued atabrine suppressive medication; observations on malaria in an infantry regiment.

    Bulletin of the Johns Hopkins Hospital·2010
    Same author

    Self-starting and self-Q-switching dynamics of passively mode-locked Nd:YLF and Nd:YAG lasers.

    Optics letters·2009
    Same author

    Passively mode-locked diode-pumped solid-state-lasers that use an antiresonant Fabry - Perot saturable absorber.

    Optics letters·2009
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    Researchers purified membrane (MLTA) and wall (WLTA) lipoteichoic acids from Streptococcus sanguis. These complex molecules contain glycerol, phosphate, glucose, and fatty acids, with MLTA showing greater dissociation in concentrated solutions.

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Lipoteichoic acids are essential cell wall components in Gram-positive bacteria.
    • Streptococcus sanguis is a common oral bacterium with implications in various infections.

    Purpose of the Study:

    • To purify and characterize membrane lipoteichoic acid (MLTA) and wall lipoteichoic acid (WLTA) from Streptococcus sanguis.
    • To elucidate the structural and aggregation properties of MLTA and WLTA.

    Main Methods:

    • Purification using Sepharose, Ecteola-cellulose, and concanavalin A-conjugated Sepharose chromatography.
    • Homogeneity assessment via disc gel electrophoresis, column chromatography, and double diffusion tests.
    • Compositional analysis and partial acid hydrolysis for structural determination.

    Related Experiment Videos

    Main Results:

    • Homogeneous MLTA and WLTA were successfully purified.
    • Both lipoteichoic acids contain glycerol, phosphate, glucose, and fatty acids in distinct ratios.
    • Structural analysis indicated a backbone of glycerol moieties linked by 1,3-phosphodiester bonds.
    • MLTA and WLTA form aggregates, with MLTA exhibiting greater dissociation.

    Conclusions:

    • The study provides a detailed characterization of MLTA and WLTA from Streptococcus sanguis.
    • The findings reveal differences in the aggregation behavior of MLTA and WLTA, potentially impacting their function.