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Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
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A promising approach for developing functional gummies with enhanced extraction and stability of anthocyanins using
Jiahao Yu1, Mengqiu Xu1, Yang Lin1
1Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Food Research International (Ottawa, Ont.)
|May 5, 2026
Summary
Natural deep eutectic solvents (NADES) with β-cyclodextrin significantly enhance anthocyanin extraction and stability for functional gummies. This green method boosts antioxidant capacity and yields more stable, functional gummies compared to traditional approaches.
Area of Science:
- Food Science
- Natural Product Chemistry
- Green Chemistry
Background:
- Anthocyanins are valuable natural pigments with antioxidant properties but suffer from poor stability.
- Developing stable, functional food products requires efficient extraction and stabilization methods for anthocyanins.
- Natural deep eutectic solvents (NADES) offer a sustainable alternative for extracting bioactive compounds.
Purpose of the Study:
- To evaluate the efficacy of NADES combined with β-cyclodextrin for anthocyanin extraction and stabilization.
- To compare the yield, stability, and antioxidant capacity of anthocyanins extracted using NADES versus traditional methods.
- To assess the suitability of the NADES-extracted anthocyanins for incorporation into functional gummies.
Main Methods:
- Extraction of anthocyanins from plant sources using a specific NADES formulation (CD-CA: β-cyclodextrin and citric acid with water).
- Comparison of anthocyanin yield and profile with traditional HCl-EtOH extraction.
- Assessment of radical scavenging capacity, photostability, and thermal stability of the extracts.
- Incorporation of Crimson Glory extract into pectin-based gummies and evaluation of anthocyanin retention and antioxidant capacity during processing and storage.
Main Results:
- The CD-CA NADES method increased anthocyanin yield by up to 39% compared to HCl-EtOH.
- Seventeen anthocyanin compounds were identified, including novel ones not found in HCl-EtOH extracts.
- CD-CA extracts showed 1.29-fold higher radical scavenging capacity and improved photostability and thermal stability.
- Anthocyanin retention in gummies was higher with the CD-CA extract (11.01% higher post-processing, 11.31% higher after 30-day storage).
- CG-CD gummies exhibited 1.32-fold increased free radical scavenging capacity.
Conclusions:
- β-cyclodextrin-based NADES provide a green and efficient method for extracting and stabilizing anthocyanins.
- This approach significantly enhances the antioxidant capacity and stability of anthocyanins in functional gummies.
- NADES represent a promising functional ingredient for developing stable, high-value food products.

