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Updated: May 6, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza
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|May 5, 2026
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Summary

A new microfluidic chip enables rapid, on-site detection of Hand, Foot, and Mouth Disease (HFMD) viruses in wastewater. This technology aids in early warning and continuous monitoring of HFMD outbreaks using wastewater-based epidemiology.

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Area of Science:

  • Environmental microbiology
  • Molecular diagnostics
  • Public health surveillance

Background:

  • Hand, Foot, and Mouth Disease (HFMD) is a prevalent childhood illness with significant epidemiological patterns.
  • Wastewater-based epidemiology offers a vital approach for tracking population-level infection dynamics and viral strains.
  • Current limitations in on-site viral detection hinder timely outbreak alerts and effective surveillance.

Purpose of the Study:

  • To develop an integrated microfluidic system for rapid, multiplexed detection of major HFMD virus subtypes in wastewater.
  • To enhance early warning systems and public health surveillance capabilities for HFMD.

Main Methods:

  • A one-pot reverse transcription recombinase polymerase amplification (RT-RPA) combined with CRISPR-Cas12a assay was optimized using heparin sodium to detect EV-A71, CV-A16, CV-A6, and CV-A10.
  • The assay was integrated into a string-powered, flywheel-type centrifugal microfluidic chip (HICAS-Chip) for automated sample processing and multiplexed detection.
  • The HICAS-Chip facilitated integrated enrichment, purification, elution, and visual nucleic acid detection within one hour.

Main Results:

  • The HICAS-Chip achieved high sensitivity (10 aM) for visual detection of nucleic acids, comparable to the one-pot assay.
  • A year-long wastewater monitoring in Guiyang City identified EV-A71 and CV-A10 as dominant HFMD subtypes, with seasonal peaks.
  • HICAS-Chip detection results demonstrated high concordance (95.83%) with standard RT-qPCR methods.

Conclusions:

  • The developed HICAS-Chip is an efficient, portable platform for on-site, multiplexed detection of HFMD viruses in wastewater.
  • This technology significantly improves wastewater-based epidemiology for early detection and continuous monitoring of HFMD trends.
  • The HICAS-Chip holds promise for enhancing infectious disease surveillance and public health response.