A two-point fluorescence detection flow cell with empirical calibration for high optical density analysis
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Updated: May 6, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Tin Weitner1,2, Davor Šakić1,2
1University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovačića 1, 10000 Zagreb, Croatia. davor.sakic@pharma.unizg.hr.
Abstract:
A two-point fluorescence detection flow cell with empirical calibration is presented for correcting inner-filter effects at high optical densities. Using only fluorescence signals from fixed detection points, linear fluorescence-concentration relationships are restored under static sample and flow conditions, demonstrating a general strategy for quantitative measurements in compact flow systems.
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