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Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: May 6, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Approaches to visualize, quantify, and manipulate phosphoinositides in cells.

Rabia Gönül¹, Agnieszka Chytła¹, Ana Miladinović²

¹Laboratory of Cancer Cell Architecture, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic.

Histochemistry and Cell Biology

|May 5, 2026

Summary

Phosphoinositides regulate cellular processes but are hard to study. This review details methods for modulating, visualizing, and quantifying these lipids to better understand their functions.

Keywords:

Cellular architecture Lipid quantification Lipid signaling Live-cell imaging Phosphoinositides Subcellular compartmentalization

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Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

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Last Updated: May 6, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Area of Science:

Cell Biology
Lipid Biochemistry

Background:

Phosphoinositides are crucial regulatory lipids controlling diverse cellular functions like membrane trafficking and gene expression.
Their compartment-specific roles are vital, but dysregulation links to diseases such as cancer and neurodegeneration.
Investigating phosphoinositides is challenging due to technical limitations in detection and manipulation.

Purpose of the Study:

To review current techniques for modulating phosphoinositide pools.
To evaluate methods for visualizing and quantifying phosphoinositides across subcellular compartments.
To provide a framework for dissecting phosphoinositide functions.

Main Methods:

Genetic manipulation: RNA interference (RNAi), CRISPR-based approaches, optogenetics.
Visualization: Fluorescent biosensors, live-cell imaging, superresolution microscopy.
Quantification: Thin-layer chromatography (TLC), mass spectrometry (MS) for species-specific profiling.

Main Results:

Current methods offer diverse capabilities for phosphoinositide research.
Genetic tools allow targeted modulation of lipid levels.
Advanced imaging and MS provide detailed spatial and molecular information.
Combining techniques enhances the ability to dissect phosphoinositide functions.

Conclusions:

A comprehensive toolkit now exists for studying phosphoinositides.
Integrating modulation, visualization, and quantification strategies is key.
This facilitates a deeper understanding of phosphoinositide roles in cellular processes and disease.