Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Netropsin, a DNA-binding oligopeptide structural and binding studies.

H M Berman, S Neidle, C Zimmer

    Biochimica Et Biophysica Acta
    |January 26, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    FKBP5 expression is related to risky foraging in house sparrows.

    Hormones and behavior·2026
    Same author

    Divergent climate impacts despite similar response to temperature in a widespread aerial insectivore.

    bioRxiv : the preprint server for biology·2025
    Same author

    Differentially spliced mitochondrial CYP419A1 contributes to ethiprole resistance in Nilaparvata lugens.

    Insect biochemistry and molecular biology·2025
    Same author

    Screening H3 Histone Acetylation in a Wild Bird, the House Sparrow (<i>Passer Domesticus</i>).

    Integrative organismal biology (Oxford, England)·2024
    Same author

    Thoracic impedance pneumography in propofol-sedated patients undergoing percutaneous endoscopic gastrostomy (PEG) placement in gastrointestinal endoscopy: A prospective, randomized trial.

    Journal of clinical anesthesia·2024
    Same author

    Reconstruction of the Corticospinal Tract in Patients with Motor-Eloquent High-Grade Gliomas Using Multilevel Fiber Tractography Combined with Functional Motor Cortex Mapping.

    AJNR. American journal of neuroradiology·2023
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Netropsin, an oligopeptide antibiotic, has a bowed crystal structure that facilitates DNA binding. This structure, along with binding data, helps explain its specificity for DNA.

    Area of Science:

    • Structural biology
    • Molecular pharmacology
    • Biochemistry

    Background:

    • Netropsin is an oligopeptide antibiotic known to interact with DNA.
    • Understanding the precise molecular interactions is crucial for antibiotic development.

    Purpose of the Study:

    • To determine the crystal structure of netropsin.
    • To investigate the binding of netropsin to DNA, specifically poly(dA) . poly(dT).
    • To elucidate the structural basis for netropsin's DNA binding specificity.

    Main Methods:

    • X-ray crystallography to determine netropsin's three-dimensional structure.
    • DNA binding studies at varying ionic strengths using poly(dA) . poly(dT).

    Main Results:

    Related Experiment Videos

  • Netropsin exhibits a bowed conformation with specific arrangements of amide, carbonyl, and methyl groups.
  • Extensive hydrogen bonding with water molecules and interactions with sulfate anions were observed.
  • Crystallographic and binding data allowed for the construction of a model explaining DNA binding specificity.

    • Conclusions:

    • The determined crystal structure of netropsin provides insights into its molecular conformation.
    • The study establishes a model for netropsin-DNA interactions, highlighting the role of its structure in binding specificity.
    • Findings contribute to understanding antibiotic-DNA interactions and potential drug design.