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Related Experiment Video

Updated: May 6, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Fast-response Phosphorescence Sensor for Dissolved Oxygen Detection Based on PtOEP/TiO₂/CB@PFFE Sensing Film.

Jia Xiao¹, Minmin Han¹, Ting Li¹

¹Department of Chemistry, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.

Journal of Fluorescence

|May 5, 2026

Summary

A novel phosphorescence sensor using a platinum octaethylporphyrin (PtOEP)/TiO2/CB@PFFE film was developed for rapid dissolved oxygen (DO) detection. This sensor offers high sensitivity and stability, making it suitable for accurate DO monitoring.

Keywords:

Dissolved oxygen sensor Fluorinated polymers PtOEP Tri-layer structure

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Area of Science:

Materials Science
Analytical Chemistry
Polymer Chemistry

Background:

Dissolved oxygen (DO) monitoring is crucial in various environmental and industrial applications.
Developing fast-response and highly sensitive DO sensors remains a significant challenge.
Phosphorescence-based sensing offers potential for sensitive and selective DO detection.

Purpose of the Study:

To develop a novel, fast-response phosphorescence sensor for dissolved oxygen (DO) detection.
To engineer a tri-layer sensing film (PtOEP/TiO2/CB@PFFE) for enhanced sensor performance.
To investigate the impact of light-scattering and light-blocking layers on sensor sensitivity and stability.

Main Methods:

Synthesized a fluorinated polymer PFFE matrix via free radical polymerization.
Fabricated a tri-layer sensing film by embedding PtOEP, TiO2, and CB within the PFFE matrix.
Characterized the sensor's response time, recovery time, reproducibility, reversibility, stability, and sensitivity using Stern-Volmer analysis.

Main Results:

The PtOEP/TiO2/CB@PFFE sensor exhibited a fast response time of 30 s and recovery time of 150 s.
The tri-layer structure significantly enhanced phosphorescence intensity and lifetime, improving detection sensitivity.
Achieved a high Stern-Volmer constant (Ksv = 0.4584 L/mg) and correlation coefficient (R = 0.9993), indicating high sensitivity for DO detection.
Demonstrated excellent reproducibility, reversibility, and stability of the sensor.

Conclusions:

The developed PtOEP/TiO2/CB@PFFE sensing film is effective for fast and highly sensitive dissolved oxygen detection.
The incorporation of light-scattering and light-blocking layers is crucial for optimizing sensor performance.
This phosphorescence sensor shows great promise for accurate and reliable DO monitoring applications.