Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Physical Methods for Controlling Microbial Growth: Temperature

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Artificial Intelligence in Embryo Selection: Current Approaches and Clinical Implications.

Bioengineering (Basel, Switzerland)·2026

Same author

A comprehensive review for defining cut-off values of oocyte, zygote, and embryo maturation arrest (OZEMA) due to maternal-effect genes: towards the establishment of clinical criteria.

Human reproduction (Oxford, England)·2026

Same author

Trial emulation for validating the clinical efficacy of a foundational AI model in embryo selection.

NPJ digital medicine·2026

Same author

Bridging the gap between embryo euploidy, pregnancy potential and morphology using artificial intelligence for ploidy estimation: a retrospective evaluation.

Reproductive biomedicine online·2026

Same author

Sperm vacuolization and mitochondrial activity in raw versus prepared semen: effect on embryological outcomes.

Reproductive biomedicine online·2026

Same author

Oocyte quality in the era of AI: integration of morphology, metabolic activity and time-lapse imaging.

Reproductive biomedicine online·2026

Search research articles

Related Experiment Video

Updated: May 7, 2026

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Media temperature control: a potentially important quality control parameter in human oocyte vitrification.

Munevver Serdarogullari^1,2,3,4, Zafer Atayurt¹, Georges Raad^5,6,7

¹Ventus IVF Center, Embryology Laboratory, Nicosia, Cyprus.

Reproduction & Fertility

|May 5, 2026

Summary

Controlling media temperature during human oocyte vitrification significantly improves embryo development. Maintaining stable temperatures enhances egg freezing success and laboratory quality control for fertility preservation.

Keywords:

embryo development oocyte vitrification pronuclear arrest quality control temperature control

More Related Videos

Minimum Volume Vitrification of Immature Feline Oocytes

Minimum Volume Vitrification of Immature Feline Oocytes

Published on: June 24, 2020

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

Related Experiment Videos

Last Updated: May 7, 2026

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Minimum Volume Vitrification of Immature Feline Oocytes

Minimum Volume Vitrification of Immature Feline Oocytes

Published on: June 24, 2020

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

Area of Science:

Reproductive Science
Cryobiology
Embryology

Background:

Human oocyte vitrification is crucial for fertility preservation and treatment.
Laboratory factors, including media temperature, can impact cryopreservation success.
Optimizing vitrification protocols is essential for improving outcomes.

Purpose of the Study:

To evaluate the role of controlled media temperature during human oocyte vitrification.
To compare fertilization, embryo development, and blastulation rates between controlled and uncontrolled temperature conditions.
To identify media temperature as a critical quality control parameter in oocyte cryopreservation.

Main Methods:

A retrospective observational study comparing two periods of oocyte vitrification: uncontrolled ambient vs. controlled media temperature.
Vitrification and equilibration solution temperatures were precisely recorded.
Embryological outcomes, including survival, fertilization, and developmental stages, were assessed.

Main Results:

Controlled temperature vitrification resulted in significantly higher temperatures (23.85°C) compared to uncontrolled (19.65°C).
No significant differences in oocyte survival or fertilization rates were observed between groups.
The uncontrolled group exhibited significantly higher developmental arrest and markedly reduced blastocyst formation compared to the controlled group.

Conclusions:

Maintaining controlled media temperature during human oocyte vitrification significantly improves pre-implantation embryo development.
Vitrification solution temperature is a critical, yet often overlooked, quality control parameter.
Implementing temperature control measures can enhance the efficacy of oocyte cryopreservation protocols.