Related Experiment Video
Updated: Jul 6, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Characterization of Grape Cane Stilbenoids and Their Photo-Degradation Products by HPLC-DAD Coupled With Trapped Ion
Paul Besrukow1, Friederike A Schnitker1, Ralf Schweiggert1
1Department of Beverage Research, Geisenheim University, Geisenheim, Germany.
Rationale:
Bioactive (E)-stilbenoids in vine extracts are prone to light-induced transformation into the respective (Z)-isomers and further degradation products, often resulting in a loss of bioactivity. We sought to study stilbenoid degradation beyond that of the well-researched resveratrol due to the high diversity of stilbenoids in nature.
Methods:
Nine authentic, individual stilbenoids and a stilbenoid-rich grape cane extract were irradiated with artificial sunlight and their photodegradation products analyzed by liquid chromatography coupled with diode array detection, electrospray ionization, trapped ion mobility spectrometry, and quadrupole time-of-flight high-resolution tandem mass spectrometry (HPLC-DAD-ESI-TIMS-QTOF-HR-MS/MS).
Results:
Overall, a total of 45 different transformation products were identified in light-exposed standard solutions and the grape cane extract. For the first time, collision cross section values of grape cane stilbenoids (154.8-286.4 Å2) and their degradation products (116.5-289.3 Å2) are reported. TIMS allowed the separation of isomeric and isobaric photodegradation products that would be challenging to differentiate when not resolved by chromatography and sharing similar HR-MS/MS fragmentation patterns, e.g., certain trihydroxyphenanthrenediones.
Conclusion:
These results highlight the potential of combining liquid chromatography, TIMS, and HR-MS/MS for these types of compounds to broaden the knowledge on photodegradation pathways of grapevine stilbenoids beyond resveratrol.
More Related Videos
Related Concept Videos
High-Resolution Mass Spectrometry (HRMS)
Mass Spectrometry: Aromatic Compound Fragmentation
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

