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Related Experiment Video

Updated: May 7, 2026

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Chamber-Specific Decellularized Extracellular Matrices Differentially Modulate Cardiomyocyte Subtypes to Drive

Dong Gyu Hwang¹, Myungji Kim², Hwanyong Choi²

¹Center for 3D Organ Printing and Stem Cells, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.

Advanced Healthcare Materials

|May 6, 2026

Summary

Decellularized extracellular matrix (dECM) from different heart chambers uniquely influences cardiomyocyte maturation. Understanding these chamber-specific cues is key for developing advanced engineered heart tissues (EHTs).

Keywords:

3D bioprinting chamber‐specific dECM decellularized extracellular matrix engineered heart tissue

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Last Updated: May 7, 2026

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Published on: March 21, 2019

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Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

Area of Science:

Biomaterials Science
Cardiovascular Research
Stem Cell Biology

Background:

Decellularized extracellular matrix (dECM) retains native tissue cues for biomaterial applications.
Regional variations within an organ's ECM are poorly understood but crucial for tissue engineering.
Induced pluripotent stem cell-derived cardiomyocyte (CM) subtypes offer potential for engineered heart tissues (EHTs).

Purpose of the Study:

To investigate the chamber-specific effects of ventricular (vtdECM) and atrial (atdECM) dECMs on engineered heart tissue (EHT) formation.
To compare the influence of vtdECM and atdECM on the maturation and function of distinct cardiomyocyte (CM) subtypes.
To explore the impact of encapsulation timing on CM maturation within different dECM environments.

Main Methods:

Proteomic analysis to characterize vtdECM and atdECM composition.
Co-culture of induced pluripotent stem cell-derived CM subtypes with chamber-specific dECMs.
Assessment of CM maturation, function, and gene expression.
Evaluation of the effects of early versus late CM encapsulation.

Main Results:

Proteomic analysis revealed distinct protein profiles for vtdECM and atdECM.
Ventricular CMs showed enhanced maturation and function in vtdECM.
Atrial CMs exhibited limited responsiveness to ECM composition, despite transcriptomic changes.
Encapsulation timing modulated structural maturation (early) and calcium handling (late).

Conclusions:

Chamber-specific dECM composition significantly impacts subtype-specific cardiomyocyte maturation and function in EHTs.
Developmental timing of encapsulation acts synergistically with ECM cues to regulate CM development.
These findings provide a foundation for designing more physiologically accurate engineered heart tissues.