Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

59.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
59.0K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.4K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.4K
Transgenic Plants02:50

Transgenic Plants

7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beauveria bassiana B094 as a dual-action biocontrol agent: Combining direct pathogenicity and endophytic colonization against Spodoptera exigua in sweet corn.

Insect science·2026
Same author

Arlocabtagene autoleucel-a GPRC5D-targeted CAR T-cell therapy in heavily pretreated relapsed/refractory multiple myeloma.

Blood·2026
Same author

Development and Validation of Prognostic Models in Patients With Stage IV Thyroid Cancer Undergoing Surgical Treatment.

The American surgeon·2026
Same author

3D transesophageal echocardiographic assessment of cusp prolapse mechanisms in tricuspid aortic root aneurysms and correlation with valve-sparing surgical outcomes.

Quantitative imaging in medicine and surgery·2026
Same author

Cerebral oximetry-guided anaesthesia and postoperative delirium in elderly patients undergoing off-pump coronary artery bypass grafting: a randomized controlled trial.

BMC geriatrics·2026
Same author

Blood Conservation Protocol for Patients Undergoing Left Ventricular Assisted Device Implantation.

Annals of cardiac anaesthesia·2026

Related Experiment Video

Updated: May 7, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

3.9K

Transposase-Assisted Donor Tethering Boosts Large-Fragment HDR in Plants.

Sha Wei1, Kai Zhang1, Shuke Deng1

  • 1School of Life Science, Shanxi University, Taiyuan, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 6, 2026
PubMed
Summary

Scientists developed a new transposase-assisted method to improve the efficiency of large DNA fragment insertion in plants using homology-directed repair (HDR). This breakthrough enhances precise gene editing for agricultural and research applications.

Keywords:
CRISPR/Cas9homology‐directed repairplanttransposase

More Related Videos

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

8.5K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

1.1K

Related Experiment Videos

Last Updated: May 7, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

3.9K
An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

8.5K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

1.1K

Area of Science:

  • Plant Biotechnology
  • Genome Engineering
  • Molecular Biology

Background:

  • Homology-directed repair (HDR) for large DNA fragment insertion in plants is often inefficient and lacks reproducibility.
  • Limited availability of donor DNA at double-strand break sites is a major obstacle for successful HDR.

Purpose of the Study:

  • To develop a novel strategy to enhance the efficiency and reliability of HDR-mediated large-fragment insertion in plants.
  • To establish a generalizable framework for precise large-fragment genome engineering in diverse plant species.

Main Methods:

  • Developed a transposase-assisted donor tethering system by fusing Cas9 to an integration-defective piggyBac variant.
  • Co-localized donor templates with Cas9-induced double-strand breaks using sequence-specific DNA-binding activity.
  • Utilized a transcription-coupled donor and a repair-pathway-biased Cas9 variant to optimize HDR frequency.

Main Results:

  • Achieved efficient and precise kilobase-scale targeted gene insertions across multiple loci in both dicot and monocot species.
  • Demonstrated that transposase-assisted donor tethering significantly improves the reliability of HDR-mediated large-fragment insertion.
  • Validated the system's effectiveness in enhancing accurate large-fragment insertions.

Conclusions:

  • Transposase-assisted donor tethering is an effective strategy for improving plant HDR efficiency.
  • The developed system provides a robust framework for precise large-fragment genome insertion in plants.
  • This advancement has significant implications for plant genetic engineering and crop improvement.