Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Preoperative neoadjuvant chemotherapy was associated with improved local control in selected cases of locally advanced or invasive oral squamous cell carcinoma: a retrospective single-center study.

Oral and maxillofacial surgery·2026

Same author

Long-Term Outcomes of Endoscopic Ultrasound-Guided Gallbladder Drainage for Acute Cholecystitis in Non-Surgical Candidates: A Multicenter Retrospective Study.

Journal of clinical medicine·2026

Same author

Second-look endoscopy does not reduce delayed bleeding after endoscopic papillectomy: a multicenter propensity score-matched analysis.

Therapeutic advances in gastroenterology·2026

Same author

Evidence-Based Clinical Practice Guidelines for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) 2026.

Hepatology research : the official journal of the Japan Society of Hepatology·2026

Same author

Varicella-Zoster Virus-Associated Esophagogastritis.

Case reports in gastrointestinal medicine·2026

Same author

CXCR2-Dependent Infiltration of Tumor-Associated Neutrophils Is Linked to Enhanced CD8<sup>+</sup> T Cell Effector Function and Reduced Lung Metastasis in 4T1 Breast Cancer.

International journal of molecular sciences·2026

Search research articles

Related Experiment Video

Updated: May 7, 2026

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Published on: November 17, 2023

Feasibility of Comprehensive Genomic Profiling for Biliary Tract Cancer Using Transpapillary Biopsy Samples: A

Kazuya Miyamoto¹, Kazuyuki Matsumoto², Toshiaki Ohara³

¹Department of Gastroenterology and Hepatology, Okayama University Hospital, Okayama, Japan.

Journal of Hepato-Biliary-Pancreatic Sciences

|May 6, 2026

Summary

Comprehensive genomic profiling (CGP) of biliary tract cancer (BTC) via transpapillary biopsy (TPB) has limited suitability, though fresh-frozen samples show higher DNA quality. Further system development for fresh-frozen samples is recommended.

Keywords:

DNA biliary tract cancer biopsy endoscopic retrograde cholangiopancreatography genetic profile

More Related Videos

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

Optimization of Breast Biopsy and Mastectomy Sample Collection Procedures for Biobanking, Personalized Medicine, and Research Applications

Optimization of Breast Biopsy and Mastectomy Sample Collection Procedures for Biobanking, Personalized Medicine, and Research Applications

Published on: September 2, 2025

Related Experiment Videos

Last Updated: May 7, 2026

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Published on: November 17, 2023

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

Optimization of Breast Biopsy and Mastectomy Sample Collection Procedures for Biobanking, Personalized Medicine, and Research Applications

Optimization of Breast Biopsy and Mastectomy Sample Collection Procedures for Biobanking, Personalized Medicine, and Research Applications

Published on: September 2, 2025

Area of Science:

Oncology
Genomics
Gastroenterology

Background:

Biliary tract cancer (BTC) patients often present with actionable mutations, making comprehensive genomic profiling (CGP) crucial for treatment decisions.
The utility of CGP using transpapillary biopsy (TPB) samples in BTC remains under investigation.

Purpose of the Study:

To evaluate the feasibility and suitability of CGP from TPB samples in patients with suspected BTC.
To compare DNA quality indicators between formalin-fixed paraffin-embedded (FFPE) and fresh-frozen (FF) samples obtained via TPB.

Main Methods:

Thirty patients with suspected BTC underwent six transpapillary biopsies using an endoscopic introducer.
Five biopsy samples were preserved as FFPE, and one as FF for DNA analysis.
Suitability for CGP was assessed using the OncoGuide NCC Oncopanel System (NCCOP) and FoundationOne CDx (F1CDx).

Main Results:

Malignancy was confirmed in 29 patients; suitability rates for CGP were 31% for NCCOP and 3.4% for F1CDx.
Fresh-frozen (FF) samples showed significantly higher DNA concentration and integrity compared to FFPE samples.
DNA quality indicators were notably superior in FF samples (p < 0.001 for concentration, p = 0.021 for integrity).

Conclusions:

Introducer-assisted multipass TPB can potentially increase the yield of adequate CGP specimens for BTC.
The suitability of TPB for CGP is limited and highly dependent on the specific genomic panel used.
Establishing protocols for utilizing FF samples is desirable due to their superior DNA quality for CGP in BTC.