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Hyperspectral tomographic diffractive microscopy: Development and applications.

Leonardo Pestana Legori¹, Carlos Alberto Chacón Ávila¹, Matthieu Debailleul¹

¹Institut de Recherche en Informatique, Mathématiques, Automatique et Signal (IRIMAS UR UHA 7499), Université de Haute-Alsace, Mulhouse, France.

Journal of Microscopy

|May 6, 2026

Summary

This summary is machine-generated.

Hyperspectral Tomographic Diffractive Microscopy (TDM) adds chemical specificity to 3D imaging by analyzing wavelength-dependent properties. This advancement enables better distinction of similar structures in biomedical and material science applications.

Keywords:

hyperspectral imaging label‐free microscopy quantitative phase imaging tomographic diffractive microscopy

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Area of Science:

Optics and Photonics
Biomedical Imaging
Materials Science

Background:

Tomographic Diffractive Microscopy (TDM) offers label-free 3D imaging beyond confocal resolution.
Previous TDM developments at IRIMAS enhanced capabilities in various configurations.
Single-wavelength TDM struggles to differentiate chemically similar structures based on optical properties.

Purpose of the Study:

To develop hyperspectral TDM for chemical specificity in 3D imaging.
To enable 3D spectroscopic characterization by exploiting wavelength-dependent optical properties.
To advance label-free imaging for complex transparent samples.

Main Methods:

Integration of a supercontinuum source for multi-wavelength TDM capability.
Development of hyperspectral synthesis techniques.
Implementation of AI-enhanced reconstruction algorithms.
Detailed description of TDM reconstruction principles.

Main Results:

Demonstration of hyperspectral TDM for chemical specificity.
Successful 3D spectroscopic characterization of samples.
Enhanced capability for distinguishing chemically distinct structures.
Potential for advanced biomedical and material imaging.

Conclusions:

Hyperspectral TDM provides crucial chemical information for 3D imaging.
The developed technology expands the application scope of TDM.
Future work includes further instrumentation, calibration, and AI integration.