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Updated: May 8, 2026

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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"1 + 1 = 3": The Added Value of Combining Immunohistochemistry and Dual-Probe Chromogen In Situ Hybridization to

Valentina Angerilli1, Alessandro Gambella2, Simona Pigozzi2

  • 1Department of Medicine (DIMED), University of Padua, Padua, Italy; Surgical Pathology Unit, ULSS2 Marca Trevigiana, Treviso, Italy.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|May 6, 2026
PubMed
Abstract

No abstract available in PubMed .

Keywords:
HER-2ancillary studycomputational pathologynext-generation pathologypredictive biomarkerprognostic biomarker

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Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...