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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Three-Dimensional Microscopy in Microbiology

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Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
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Updated: May 8, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Structured Illumination Microscopy.

Andrew Thompson1, Rumelo Amor2

  • 1Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2026
PubMed
Summary
This summary is machine-generated.

Structured illumination microscopy (SIM) offers super-resolution imaging by using patterned light, doubling optical resolution without special dyes. This guide details SIM implementation, acquisition, processing, and artifact management for fluorescence microscopy.

Keywords:
Fluorescence microscopyStructured illumination microscopySuper-resolution microscopyVolumetric imaging

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Last Updated: May 8, 2026

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Published on: May 30, 2016

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Area of Science:

  • Optical Microscopy
  • Super-resolution Imaging
  • Biophysics

Background:

  • Standard optical microscopy is limited by diffraction.
  • Super-resolution techniques overcome these limits.
  • Structured illumination microscopy (SIM) is a key super-resolution method.

Purpose of the Study:

  • To provide a comprehensive guide to structured illumination microscopy (SIM).
  • To explain the principles and implementation of SIM in fluorescence microscopy.
  • To address practical aspects of SIM acquisition, processing, and artifact correction.

Main Methods:

  • Detailed explanation of structured illumination principles.
  • Step-by-step guide to SIM data acquisition.
  • Description of image reconstruction and processing algorithms.
  • Identification and analysis of common SIM artifacts.

Main Results:

  • Demonstration of achieving twice the diffraction-limited resolution.
  • Practical insights into optimizing SIM experiments.
  • Strategies for mitigating common acquisition and processing artifacts.
  • Guidance on interpreting SIM data accurately.

Conclusions:

  • SIM is a powerful, accessible super-resolution technique.
  • Proper implementation and processing are crucial for high-quality SIM images.
  • Understanding and addressing artifacts enhances SIM reliability and data interpretation.