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Updated: May 8, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
1H R1ρ relaxation identifies a hidden intermediate in DNA base-pairing
Rubin Dasgupta1,2, Christian Steinmetzger1,2, Julian Ilgen1,3
1Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
None:
1H R1ρ Relaxation dispersion (RD) NMR experiments provide valuable atomic-level insights into transient, high-energy conformational states of biomolecules. However, cross-relaxation artifacts can hamper its interpretation and therefore limiting broader adoption. This study explicitly quantifies cross-relaxation effects on 1H R1ρ relaxation rates, extending the general applicability of ¹H R1ρ to probe dynamics at natural abundance. Artifacts were found to be negligible for neighbouring dipolar-coupled protons, >3 Å apart, and a concept for identification for protons less than 3 Å is provided. This approach revealed a second excited state (ES2) in DNA base-pairing that extends the well-established Watson-Crick-Franklin (WCF) ground state (GS) - Hoogsteen (HG) equilibrium. A structural model for ES2 is proposed based on evidence from 1H R1ρ RD, trapping via DNA modifications, metadynamics simulations, and DFT-based chemical shift calculations. ES2 was stabilised by the anticancer drug Actinomycin D, providing direct experimental evidence that small molecule can remodel conformational landscape of DNA. Together, these results demonstrate both a methodological advance by establishing reliable conditions for 1H R1ρ RD studies, and a mechanistic discovery of a drug-stabilised intermediate in DNA base-pairing dynamics.
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