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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Related Experiment Video

Updated: May 8, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

RNA-triggered cell killing with CRISPR-Cas12a2.

Paul Scholz1, Jared Thompson2, Kadin T Crosby3

  • 1Akribion Therapeutics GmbH, Zwingenberg, Germany. paul.scholz@akribion-therapeutics.com.

Nature
|May 6, 2026
PubMed
Summary
This summary is machine-generated.

Scientists have discovered a new CRISPR system, Cas12a2, that can eliminate specific yeast and human cells. This RNA-guided DNA shredding technology offers precise cell elimination based on target gene activity.

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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

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Last Updated: May 8, 2026

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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Targeted cell elimination is crucial across various scientific fields.
  • CRISPR nucleases are effective for bacterial counterselection but limited in eukaryotes.
  • Cas12a2 is a novel CRISPR nuclease with RNA-triggered DNA shredding capabilities.

Purpose of the Study:

  • To investigate the potential of Cas12a2 for programmable, sequence-specific cell elimination in eukaryotes.
  • To demonstrate Cas12a2's ability to induce cell death via DNA breaks in trans.
  • To explore Cas12a2 applications in eliminating cells based on specific transcripts.

Main Methods:

  • Utilized the Cas12a2 nuclease system.
  • Activated Cas12a2 with specific RNA triggers to induce DNA shredding.
  • Applied the system to yeast and human cells expressing target transcripts.
  • Assessed cell viability and off-target effects.

Main Results:

  • Cas12a2 demonstrated RNA-triggered, sequence-specific DNA shredding.
  • Activation of Cas12a2 led to double-stranded DNA breaks and cell death in target cells.
  • The system successfully eliminated cells harboring human papillomavirus, failed gene editing, or KRAS oncogenic mutations.
  • No off-target activation was observed.

Conclusions:

  • Cas12a2 enables programmable and sequence-specific elimination of eukaryotic cells based on transcriptional profiles.
  • This expands the CRISPR toolbox for applications in research, medicine, and biotechnology.
  • Cas12a2 offers a novel approach for targeted cell ablation with high specificity.