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Related Experiment Video

Updated: May 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Precise 3D Tracking of Highly Non-Planar Eukaryotic Flagellar Beating Patterns Using Digital Holographic Microscopy.

Patryk Nienaltowski1,2, Jonasz Słomka1,3, Federica Miano4

  • 1Institute of Environmental Engineering, ETH Zürich, Zürich, Switzerland.

Small Methods
|May 7, 2026
PubMed
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We developed holoV3C, a novel method for precise 3D tracking of eukaryotic flagella. This technique enables detailed analysis of flagellar movement, advancing our understanding of cellular motility and microorganism dynamics.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Accurate 3D tracking of eukaryotic flagella is crucial for understanding cellular motility.
  • Existing methods struggle with precise reconstruction of highly non-planar flagellar beating patterns.

Purpose of the Study:

  • To present holoV3C, a new method for label-free, high-resolution 3D tracking of eukaryotic flagella.
  • To enable precise kinematic reconstruction of complex and non-planar flagellar movements.

Main Methods:

  • Digital Holographic Microscopy (DHM) combined with phase anomaly detection.
  • Computationally efficient algorithm for high temporal and axial resolution tracking.
  • Validation using mouse sperm flagella and the protist Reclinomonas americana.
Keywords:
3D trackingbiophysicsdigital holographic microscopyflagellar kinematics

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Biophysical Characterization of Flagellar Motor Functions
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Biophysical Characterization of Flagellar Motor Functions

Published on: January 18, 2017

Related Experiment Videos

Last Updated: May 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Biophysical Characterization of Flagellar Motor Functions
06:08

Biophysical Characterization of Flagellar Motor Functions

Published on: January 18, 2017

Main Results:

  • Achieved 0.25 µm axial resolution for mouse sperm flagella and 53 nm for particles.
  • Successfully reconstructed highly non-planar flagellar dynamics at 200 frames per second.
  • Demonstrated label-free, high-resolution 3D tracking across large sampling volumes.

Conclusions:

  • holoV3C provides precise, label-free 3D tracking of non-planar eukaryotic flagella.
  • This method offers significant insights into flagellar dynamics and microorganism motility.
  • Opens new research avenues in microbial ecology and cell function.