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Updated: May 8, 2026
08:27
A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
Published on: May 22, 2019
XIST Is a Key Modulator Associated with the Adhesome Network
Biorxiv : the Preprint Server for Biology
|May 7, 2026
Summary
The X-inactivation specific transcript (XIST) long non-coding RNA regulates cell adhesion and stemness. XIST modulates the adhesome network, impacting cancer cell plasticity and clinical outcomes.
Area of Science:
- Epigenetics
- Cancer Biology
- Molecular Biology
Background:
- The X-inactivation specific transcript (XIST) long non-coding RNA is crucial for X chromosome inactivation and dosage compensation.
- While XIST's role in development is established, emerging evidence suggests non-canonical functions in cancer stemness and cellular plasticity.
- The adhesome network, comprising cell adhesion molecules, is integral to regulating cancer stemness.
Purpose of the Study:
- To investigate the previously unrecognized link between XIST and the adhesome network.
- To explore XIST's role in regulating cell adhesion pathways and its association with cancer stemness.
Main Methods:
- Gene expression and gene ontology analysis were performed on XIST-knockdown ovarian cancer cells.
- Genotype-Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA) datasets were utilized to analyze XIST and adhesome gene correlations.
- Network analysis and hazard ratio analysis were employed to assess interactions and clinical implications.
Main Results:
- XIST knockdown significantly altered adhesome gene expression and downstream adhesion pathways in ovarian cancer cells.
- Distinct correlations between XIST lncRNA and adhesome genes were identified across normal and cancer tissues, associated with cell stemness.
- Network analysis suggested potential nuclear interactions between XIST and specific adhesome genes, with functional implications for clinical outcomes.
Conclusions:
- XIST acts as a modulator strongly associated with the adhesome network and cell stemness.
- A novel link between lncRNA-mediated epigenetic regulation of cell adhesion genes and cancer stemness is supported.
- XIST is highlighted as a key regulator contributing to the adhesome network, with potential implications for cancer development and progression.
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