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Related Experiment Video

Updated: May 8, 2026

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

Fibroblast Growth Factor Receptor Signaling in Maturing Osteoblasts Controls Cell-Matrix Interactions Critical for

Debabrata Patra1, Craig Smith1, Chenming Wei2

  • 1Department of Developmental Biology, Washington University School of Medicine and McKelvey School of Engineering, Washington University, St. Louis, MO 63110 USA.

Biorxiv : the Preprint Server for Biology
|May 7, 2026
PubMed
Summary

Fibroblast growth factor receptor 1 (FGFR1) signaling is essential for osteocyte survival and bone integrity. Its deficiency leads to osteocyte death, disrupted bone matrix, and impaired communication, impacting skeletal homeostasis.

Keywords:
ApoptosisBone MatrixCollagenOsteoblastOsteocalcinOsteocyteOsteoidPodoplanin

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Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

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Last Updated: May 8, 2026

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
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Published on: November 13, 2023

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Area of Science:

  • Bone Biology
  • Cellular Differentiation
  • Skeletal Homeostasis

Background:

  • Osteocytes, the most abundant cells in cortical bone, are crucial for skeletal homeostasis.
  • Osteocyte loss is linked to bone aging and fragility.
  • Mechanisms regulating osteocyte formation and survival are not well understood.

Purpose of the Study:

  • To investigate the role of fibroblast growth factor receptor 1 (FGFR1) signaling in osteocyte viability and bone integrity.
  • To elucidate the mechanisms by which FGFR1 signaling influences osteoblast-to-osteocyte differentiation and survival.

Main Methods:

  • Inactivation of FGFR1 in mature osteoblast lineage.
  • Lineage tracing and analysis of bone formation.
  • RNA sequencing of cortical bone.
  • In vitro studies using Ocy454 cells.

Main Results:

  • FGFR1 inactivation led to extensive osteocyte death and impaired survival of newly embedded osteocytes.
  • Defects included ectopic gene expression, abnormal lacunae, defective dendrite formation, and disrupted canalicular networks.
  • RNA sequencing revealed reduced expression of extracellular matrix and neuronal regulatory genes.
  • Histological and ultrastructural analyses showed disorganized collagen, diminished osteoid, and abnormal mineralization.

Conclusions:

  • FGFR1 signaling is essential for osteocyte viability, dendrite formation, and bone integrity.
  • FGFR1 deficiency disrupts bone extracellular matrix organization and cell communication, leading to osteocyte death.
  • FGFR signaling plays a dual role in regulating matrix-dependent and intrinsic cell differentiation during osteocyte formation, crucial for bone homeostasis.