Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 8, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

All-in-one, Cas13d-based cell-specific gene knockdown system for zebrafish.

Dongeun Heo1, Cody L Call1, Jiakun Chen1

  • 1Vollum Institute, Oregon Health & Science University, Portland, Oregon, USA.

Biorxiv : the Preprint Server for Biology
|May 7, 2026
PubMed
Summary

Researchers developed a new CRISPR-Cas13d tool for precise gene knockdown in specific zebrafish cells. This RNA-targeting method efficiently alters gene function, aiding neural circuit and glial biology research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developmental and age-related synapse elimination is mediated by glial Croquemort.

Neuron·2026
Same author

Diet-dependent sleep modulation by the <i>Drosophila</i> amino acid transporter ANIDRA.

bioRxiv : the preprint server for biology·2026
Same author

PIEZOs regulate oligodendrocyte sheath formation, expansion, and myelination potential.

bioRxiv : the preprint server for biology·2026
Same author

Flexible ensheathment of axons enables myelination of complex CNS networks.

Nature·2026
Same author

Conservation of Neuron-Astrocyte Correlated Activity in Developing Sensory Pathways.

Glia·2026
Same author

Pre-myelinating oligodendrocyte ADGRG1 is required for axon ensheathment and CNS myelin formation.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Cell type-specific genetic manipulation is crucial for understanding neural circuits and glial biology.
  • Existing methods for targeted gene knockdown in zebrafish are limited, hindering research progress.

Purpose of the Study:

  • To introduce and validate a novel CRISPR-Cas13d-based mRNA knockdown platform for zebrafish.
  • To enable efficient and flexible gene function perturbation in specific cell types.

Main Methods:

  • Engineered an all-in-one vector for cell type-specific RfxCas13d expression and crRNA delivery.
  • Validated the platform across astrocytes, oligodendrocytes, and microglia in the zebrafish CNS.
  • Utilized RNA targeting (CRISPR-Cas13d) instead of DNA editing.

More Related Videos

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Related Experiment Videos

Last Updated: May 8, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Main Results:

  • Achieved consistent target mRNA depletion using Cas13d-mediated knockdown.
  • Observed heritable and reproducible morphological and functional phenotypes.
  • Demonstrated the robustness and efficiency of the RNA-targeting approach.

Conclusions:

  • The CRISPR-Cas13d platform provides a reliable, efficient, and scalable method for RNA-level gene perturbation in zebrafish.
  • This approach complements existing genome-editing tools like CRISPR-Cas9.
  • Expands zebrafish genetic technologies for in vivo cell type-specific studies and genetic screens.