Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Dosage Compensation

Dosage Compensation

In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

F-actin organization excludes E-cadherin from the division furrow to ensure cytokinesis fidelity.

Life science alliance·2026

Same author

<i>P. aeruginosa</i> disrupts ARP-2/3 mediated apical F-actin organization to induce intestinal deformation in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2025

Same author

Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset.

Nature communications·2018

See all related articles

Search research articles

Related Experiment Video

Updated: May 8, 2026

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Published on: April 19, 2018

TOCA-2 regulates gonad development in C. elegans.

Yogesh Pratap, Tanushree Sinha, Anup Padmanabhan

Biorxiv : the Preprint Server for Biology

|May 7, 2026

Summary

TOCA-2 is crucial for maintaining C. elegans gonad structure and function. Loss of TOCA-2 disrupts the actomyosin corset, impairing germline integrity and cytoplasmic flow during development.

Area of Science:

Developmental Biology
Cell Biology
Molecular Biology

Background:

Gonad development in C. elegans relies on complex biochemical and physical interactions.
The specific roles of many factors, including TOCA-2 (Transducer of CDC42 Dependent Actin Assembly), are not fully understood.
TOCA-2 is an F-BAR protein involved in membrane remodeling and actin cytoskeleton regulation during oogenesis and embryogenesis.

Purpose of the Study:

To investigate the role of TOCA-2 in the structural integrity and function of the C. elegans gonad.
To elucidate the mechanisms by which TOCA-2 influences gonad morphogenesis and maintenance.

Main Methods:

Analysis of toca-2(null) mutant animals.
Phalloidin staining to visualize actin cytoskeleton.

More Related Videos

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Published on: July 30, 2018

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

Related Experiment Videos

Last Updated: May 8, 2026

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Published on: April 19, 2018

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Published on: July 30, 2018

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

Cytoplasmic particle velocity analysis to assess cytoplasmic flow.

Main Results:

toca-2(null) mutants display significant gonad architectural defects, particularly on the dorsal side.
The actomyosin corset surrounding the common rachis is severely disorganized in mutants.
Disorganization of the actomyosin corset leads to disrupted cytoplasmic flow within the syncytial germline.

Conclusions:

TOCA-2 is essential for maintaining C. elegans gonad structural integrity and function.
TOCA-2 regulates gonad morphogenesis and maintenance by organizing the actomyosin cytoskeleton and ensuring proper cytoplasmic flow.
This study provides insights into TOCA-2's role in broader developmental pathways involving actomyosin dynamics.