Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 8, 2026

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Published on: March 24, 2022

A Head and Neck Cancer Patient-Specific Microphysiological System for Predicting Response to Chemoradiation.

Adeel Ahmed¹, Nathan Hendrikse¹, Rene Welch Schwartz²

¹Carbone Cancer Center, University of Wisconsin - Madison, Madison, WI, USA.

Biorxiv : the Preprint Server for Biology

|May 7, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

In situ vaccination, low-dose targeted radionuclide therapy, and immune checkpoint inhibition eradicate poorly immunogenic metastatic tumors in murine cancer models.

Cancer immunology research·2026

Same author

CXCL13-CXCR5 signaling in CD8<sup>+</sup> T cell recruitment and lymphoid immune organization in clear cell renal cell carcinoma.

Cancer immunology, immunotherapy : CII·2026

Same author

CXCL13-CXCR5 Signaling in CD8<sup>+</sup> T Cell Recruitment and Lymphoid Immune Organization in Clear Cell Renal Cell Carcinoma.

bioRxiv : the preprint server for biology·2026

Same author

Lymphatic Endothelial Cells Regulate Neutrophil Phenotypes and Function in a Microphysiological Model of Infection.

bioRxiv : the preprint server for biology·2026

Same author

Integrated Multiomic Profiling Identifies BRD8/EP400 as a Pivotal Chromatin Module Mediating Anti-HER2 Response in HR+/HER2+ Breast Cancer.

Cancer research·2026

Same author

Integrated Single-Cell and Spatial Analysis Reveals Context-Dependent Myeloid-T Cell Interactions in Response to Immune Checkpoint Blockade in Head and Neck Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

A novel patient-specific microphysiological system (MPS) models head and neck cancer (HNC) tumor microenvironments. This system accurately predicts treatment response, offering a functional tool for personalized HNC therapy.

Area of Science:

Oncology
Biotechnology
Cancer Research

Background:

Head and neck cancer (HNC) is a prevalent malignancy with high recurrence rates.
Current predictive models for HNC treatment response lack patient-specific complexity and tumor microenvironment (TME) representation.
Chemoradiation is standard care, but predicting individual patient response remains challenging.

Purpose of the Study:

To develop and validate a patient-specific microphysiological system (MPS) for reconstructing the HNC TME.
To assess the MPS's ability to model HNC biology, including hypoxia and radiation response.
To identify functional metrics within the MPS that predict therapeutic response and clinical outcomes.

Main Methods:

Constructed a vascularized 3D MPS using patient-derived HNC cells, fibroblasts, and immune cells within a hydrogel.

More Related Videos

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Published on: April 20, 2018

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Published on: April 23, 2021

Related Experiment Videos

Last Updated: May 8, 2026

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Published on: March 24, 2022

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Published on: April 20, 2018

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Published on: April 23, 2021

Utilized single-cell RNA sequencing to confirm preservation of cellular heterogeneity.

Employed compartment-resolved imaging and spheroid analysis to monitor treatment dynamics.

Main Results:

The MPS successfully recapitulated HNC TME characteristics, including tumor hypoxia and altered radiation response.
Patient-specific MPS demonstrated divergent responses to chemoradiation, reflecting clinical heterogeneity.
The rate of spheroid area change during treatment correlated with tumor recurrence, indicating predictive potential.

Conclusions:

The patient-specific MPS is a viable platform for modeling HNC TME and predicting chemoradiation response.
This system offers a functional tool to overcome limitations of current predictive models for HNC.
Spheroid area change dynamics in the MPS may serve as a novel biomarker for HNC therapeutic response and recurrence.