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H2S-Amplified "Three-in-One" Antibacterial Strategy for Periodontitis Treatment Using a Photosensitive
Shuang Liang1,2, Meng-Hao Li2, Liang Cheng1
1Department of Prosthodontics, Jilin Provincial Key Laboratory of Sciences and Technology for Stomatology Nanoengineering, School and Hospital of Stomatology, Jilin University, Changchun, P. R. China.
Summary
This study introduces a novel nanomedicine platform that enhances reactive oxygen species (ROS) to combat biofilm infections by disabling antioxidant defenses. The material shows significant antibacterial activity and biofilm eradication.
Area of Science:
- Biomedical Engineering
- Materials Science
- Nanotechnology
Background:
- Reactive oxygen species (ROS)-based nanomedicine shows promise for biofilm-associated infections (BAIs).
- Antioxidant systems in biofilms reduce ROS effectiveness.
- Combining ROS generation with antioxidant pathway inhibition is a potential strategy.
Purpose of the Study:
- To develop a composite platform for synergistic treatment of BAIs.
- To enhance ROS generation and inhibit local antioxidant pathways.
- To investigate the antibacterial mechanism and efficacy of the novel material.
Main Methods:
- Fabrication of a composite platform (COF/HKUST-10) using covalent organic frameworks and a copper-based metal-organic framework.
- Activation of the platform by endogenous hydrogen sulfide and exogenous light.
- Transcriptomic analysis to elucidate the antibacterial mechanism against Porphyromonas gingivalis.
- In vitro and in vivo experiments to evaluate antibacterial activity, biofilm eradication, and anti-inflammatory effects.
Main Results:
- The COF/HKUST-10 platform demonstrated synergistic catalytic reactions enhancing ROS generation and antioxidant inhibition.
- Transcriptomic analysis revealed disruption of porphyrin metabolism in P. gingivalis by ROS and copper ions, impairing energy metabolism and pathogenicity.
- The material exhibited robust antibacterial activity, efficient biofilm eradication, and significant inflammation reduction.
- The synergistic strategy involved ROS amplification, antioxidant system depletion, and copper ion-mediated bactericidal effects.
Conclusions:
- The developed COF/HKUST-10 platform offers a novel "three-in-one" synergistic antibacterial strategy for treating BAIs.
- This approach effectively overcomes biofilm antioxidant defenses and enhances therapeutic outcomes.
- The study broadens the application of supramolecular materials in combating challenging infections.
